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BIOINF 07-012: PCR Survey of <i>Xenoturbella bocki</i> Hox Genes

Guido Fritzsch, Manja U. Böhme, Mike Thorndyke, Hiroaki Nakano, Olle Israelsson, Thomas Stach, Martin Schlegel, Thomas Hankeln, Thomas Stach, Peter F. Stadler

Neighbor Joining Trees of NCBI tree-reconstruction service

  • XbHox1-Tree
  • XbHoxM1a-Tree
  • XbHoxM1b-Tree
  • XbHoxM2-Tree
  • XbHoxP-Tree

    • List of the used primer in this work

    HoxF1 (Hano et al. 2001)CARYTNCANGARYTNGARAA
    HoxF2 (Hano et al. 2001)YTNGARYTNGARAARGARTT
    HoxR1 (Hano et al. 2001)TTCATNCKNCKRTTYTGRAA
    HoxR2 (Hano et al. 2001)CKRTTYTGRAACCADATYTT
    FHox3 (Peterson et al. 2004)AAYMRNTAYYTNCARAARCA
    FHox2/3 (Peterson et al. 2004)AAYMRNTAYYTNTGYMRNCC
    EN-F (Hano et al. 2001)GAYGARAARMGNCCNMG

    The Hano et al. (2001) primer set used in this work is a more degenerate version of the Hox primers proposed by Mito and Endo (1997) in a PCR survey of a sea star and later used successfully in a hemichordate by Peterson (2004). Additional we used the Hox primer proposed by Mito and Endo (2000).