Publications - Published papers
Please find below publications of our group. Currently, we list 565 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (
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webmaster@bioinf.uni-leipzig.de
) is restricted to our local network and chosen collaborators.
If you have problems accessing electronic information, please let us know:webmaster@bioinf.uni-leipzig.de
©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.
Temperature-responsive in vitro RNA structurome of Yersinia pseudotuberculosis.
Righetti, Francesco and Nuss, Aaron M. and Twittenhoff, Christian and Beele, Sascha and Urban, Kristina and Will, Sebastian and Bernhart, Stephan H. and Stadler, Peter F. and Dersch, Petra and Narberhaus, Franz
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PREPRINT 16-001: [ Supplement ]
Status: Published
Proceedings of the National Academy of Sciences of the United States of America 113 (26): 7237--7242
Abstract
RNA structures are fundamentally important for RNA function. Dynamic, condition-dependent structural changes are able to modulate gene expression as shown for riboswitches and RNA thermometers. By parallel analysis of RNA structures, we mapped the RNA structurome of Yersinia pseudotuberculosis at three different temperatures. This human pathogen is exquisitely responsive to host body temperature (37 {\textdegree}C), which induces a major metabolic transition. Our analysis profiles the structure of more than 1,750 RNAs at 25 {\textdegree}C, 37 {\textdegree}C, and 42 {\textdegree}C. Average mRNAs tend to be unstructured around the ribosome binding site. We searched for 5'-UTRs that are folded at low temperature and identified novel thermoresponsive RNA structures from diverse gene categories. The regulatory potential of 16 candidates was validated. In summary, we present a dynamic bacterial RNA structurome and find that the expression of virulence-relevant functions in Y. pseudotuberculosis and reprogramming of its metabolism in response to temperature is associated with a restructuring of numerous mRNAs.