Publications - Published papers
Please find below publications of our group. Currently, we list 565 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (
) is restricted to our local network and chosen collaborators.
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) is restricted to our local network and chosen collaborators.
If you have problems accessing electronic information, please let us know:©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.
The Coilin Interactome Identifies Hundreds of Small Noncoding RNAs that Traffic through Cajal Bodies
Martin Machyna, Stephanie Kehr, Korinna Straube, Dennis Kappei, Frank Buchholz, Falk Butter, Jernej Ule, Jana Hertel, Peter F. Stadler, Karla M. Neugebauer
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Status: Published
Molecular Cell,Volume 56, Issue 3, 6 November 2014, Pages 389–399
Abstract
Coilin protein scaffolds Cajal bodies (CBs)—subnuclear
compartments enriched in small nuclear RNAs
(snRNAs)—and promotes efficient spliceosomal
snRNP assembly. The molecular function of coilin,
which is intrinsically disordered with no defined
motifs, is poorly understood. We use UV crosslinking
and immunoprecipitation (iCLIP) to determine
whether mammalian coilin binds RNA in vivo and
to identify targets. Robust detection of snRNA
transcripts correlated with coilin ChIP-seq peaks
on snRNA genes, indicating that coilin binding to
nascent snRNAs is a site-specific CB nucleator.
Surprisingly, several hundred small nucleolar RNAs
(snoRNAs) were identified as coilin interactors,
including numerous unannotated mouse and human
snoRNAs. We show that all classes of snoRNAs
concentrate in CBs. Moreover, snoRNAs lacking
specific CB retention signals traffic through CBs en
route to nucleoli, consistent with the role of CBs in
small RNP assembly. Thus, coilin couples snRNA
and snoRNA biogenesis, making CBs the cellular
hub of small ncRNA metabolism.
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