Publications - Published papers
Please find below publications of our group. Currently, we list 565 papers. Some of the publications are in collaboration with the group of Sonja Prohaska and are also listed in the publication list for her individual group. Access to published papers (
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) is restricted to our local network and chosen collaborators.
If you have problems accessing electronic information, please let us know:webmaster@bioinf.uni-leipzig.de
©NOTICE: All papers are copyrighted by the authors; If you would like to use all or a portion of any paper, please contact the author.
Deletion analysis of the 3' long terminal repeat sequence of plant retrotransposon Tto1 identifies 125 base pairs as sufficient for first strand transfer
Andrea Tramontano, Alexander Donath, Stefan H. Bernhart, Kristin Reiche, Gudrun Böhmdorfer, Peter F. Stadler, and Andreas Bachmair
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Status: Published
Virology (2011)
Abstract
Retroviruses and many retrotransposons are flanked by sequence repeats
called long terminal repeats (LTRs). These sequences contain a promoter
region, which is active in the 5' LTR, and transcription termination signals,
which are active in the LTR copy present at the 3' end. In addition, the LTR
has an essential role in reverse transcription. A part of the LTR, the
redundancy region (R), is present at both ends of viral / element mRNA and
serves as the sequence where the emerging first strand of the cDNA changes
template, switching from the 5' end of mRNA to the 3' end of Tto1 mRNA.
Here we show that, in the primordial retrotransposon Tto1, the promoter and
terminator functions of the LTR can be supplied by heterologous sequences,
thereby converting the LTR into a significantly shorter sub-terminal repeat. An
engineered Tto1 element with 125 instead of the usual 574 base pairs
repeated in the 5' and 3' region can still promote strand transfer during cDNA
synthesis, defining a minimal R region for this element. Based on this finding,
we propose a model for first strand transfer of Tto1.
Keywords
long terminal repeat (LTR) retrotransposon, Tto1
Note
10.1016/j.virol.2010.12.059