CLUSTAL W(1.81) multiple sequence alignment 489526_ENSMUSG00000042744_MOUSE_3011_3232/1-224 -ACTCACCTAGTTTGATGTGGACTTTGTCTGTGGAAAGAACCACAGAAGGGTACTGGTCA 489526_ENSDARG00000003682_ZEBRAFISH_7956_8073/1-224 T----------------------------------------------------------- 489526_ENSRNOG00000001352_RAT_1589_1811/1-224 -ACTCACCTAGTTTTATGTGGACTTTGTCTGTGGAGAGAACCACAGAAGGGTACTGGTCA 489526_SINFRUG00000133157_FUGU_749_866/1-224 G----------------------------------------------------------- 489526_ENSMUSG00000042744_MOUSE_3011_3232/1-224 GCTGTGGGTGGAGGGGCGAGCCCAGTGTTGGCTGGGGAGGCGTCTCGAGGGTAGCAGGCA 489526_ENSDARG00000003682_ZEBRAFISH_7956_8073/1-224 ----------------------------------------------GAGCACAG-AGGTG 489526_ENSRNOG00000001352_RAT_1589_1811/1-224 GCTGTAGGTGGAGGGGCGAGCCCAGTGTTGGCTGGGGAGGCGTCTCGAGGGTAGCAGGCG 489526_SINFRUG00000133157_FUGU_749_866/1-224 ----------------------------------------------CAGGACAG-ACGGA ** ** * * 489526_ENSMUSG00000042744_MOUSE_3011_3232/1-224 GCCTCAATGAGGTTCAGCTGGCCGGTACGCTTCACTTTGTGGCCAAGCCCATAGACGAGC 489526_ENSDARG00000003682_ZEBRAFISH_7956_8073/1-224 GGCACCGTGTCCAGCTCATCCTCAGAGCGCTCAACGGTCTCTCCTACACTGTAGGTTAGC 489526_ENSRNOG00000001352_RAT_1589_1811/1-224 GCTTCAATGAGGTTCAGCTGGCCAGTGCGCTTCACTTTGTGGCCGAGCCCATAGACGAGC 489526_SINFRUG00000133157_FUGU_749_866/1-224 GGCACCGTGTCCGGCTCGTCTTCGGAGCGCTCGCTGCTCTCGTCAAGCTTCCACGTTAGC * * ** * * * * **** * * * * * *** 489526_ENSMUSG00000042744_MOUSE_3011_3232/1-224 ACGCGTGCCCACGGAGCCTTGTACAAAGAGGAGCTGAGAGGAA- 489526_ENSDARG00000003682_ZEBRAFISH_7956_8073/1-224 AGCTGTTCAGCGAAGGCCATCGCCAATGGAAACTCAAGAGGCAA 489526_ENSRNOG00000001352_RAT_1589_1811/1-224 ACACGTGCCCACGGAGCCTTGTACAGAGAGGAGCTGAGAGGAAG 489526_SINFRUG00000133157_FUGU_749_866/1-224 AACTGTTCGGCAAAAGCAATGGCCAAAGGGAACTCCAGCGGCAG * ** * ** * ** * * ** ** *
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 224 Mean pairwise identity: 41.39 Mean single sequence MFE: -66.55 Consensus MFE: -11.94 Energy contribution: -15.38 Covariance contribution: 3.44 Mean z-score: -1.22 Structure conservation index: 0.18 SVM decision value: -0.05 SVM RNA-class probability: 0.510424 Prediction: RNA ###################################################################### >489526_ENSMUSG00000042744_MOUSE_3011_3232/1-224 ACUCACCUAGUUUGAUGUGGACUUUGUCUGUGGAAAGAACCACAGAAGGGUACUGGUCAGCUGUGGGUGGAGGGGCGAGCCCAGUGUUGGCUGGGGAGGCGUCUCGAGGGUAGCAGGCAGCCUCAAUGAGGUUCAGCUGGCCGGUACGCUUCACUUUGUGGCCAAGCCCAUAGACGAGCACGCGUGCCCACGGAGCCUUGUACAAAGAGGAGCUGAGAGGAA .(((............((((...((((((((((......(((((((((.((((((((((((((......((((.((...((((((....))))))...)).))))((((.(.(....)).)))).........)))))))))))))).))))....)))))......))))))))))(((....)))))))((..((((.......))))..))..)))... ( -93.70) >489526_ENSDARG00000003682_ZEBRAFISH_7956_8073/1-224 UGAGCACAGAGGUGGGCACCGUGUCCAGCUCAUCCUCAGAGCGCUCAACGGUCUCUCCUACACUGUAGGUUAGCAGCUGUUCAGCGAAGGCCAUCGCCAAUGGAAACUCAAGAGGCAA ...((...(((.(((((.....))))).)))...(((.(((((((.((((((((..(((((...)))))..))..)))))).))))..(((....)))........)))..))))).. ( -41.60) >489526_ENSRNOG00000001352_RAT_1589_1811/1-224 ACUCACCUAGUUUUAUGUGGACUUUGUCUGUGGAGAGAACCACAGAAGGGUACUGGUCAGCUGUAGGUGGAGGGGCGAGCCCAGUGUUGGCUGGGGAGGCGUCUCGAGGGUAGCAGGCGGCUUCAAUGAGGUUCAGCUGGCCAGUGCGCUUCACUUUGUGGCCGAGCCCAUAGACGAGCACACGUGCCCACGGAGCCUUGUACAGAGAGGAGCUGAGAGGAAG .(((.........((((((..(((.((((((((.(....(((((((((.((((((((((((((......((((.((...((((((....))))))...)).))))((((...(....)..)))).........)))))))))))))).))))....))))).....))))))))))))..))))))....(((..((((.......))))..))).))).... ( -92.50) >489526_SINFRUG00000133157_FUGU_749_866/1-224 GCAGGACAGACGGAGGCACCGUGUCCGGCUCGUCUUCGGAGCGCUCGCUGCUCUCGUCAAGCUUCCACGUUAGCAACUGUUCGGCAAAAGCAAUGGCCAAAGGGAACUCCAGCGGCAG ((.(((((((((..((((.((.((...((((.......)))))).)).))))..))))..(((........)))...))))).))..........(((....((....))...))).. ( -38.40) >consensus __________________________________________________________________________________________________________GAGGACAG_AGGCAGCCACAAUGACCUGCACCUCGCCAGAGCGCUCCACGUUCUCGCCAAGCCCAUAGACGAGCACCCGUGCCCACAAAGCCAUGGACAAAGAGAACCCGAGAGGAAG ...........................(((((((......))))))).........................((((((..(((((......)))))....))))))............((((................(((((((((((.....)))))))))))........................((((......)))).........))))........ (-11.94 = -15.38 + 3.44)
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