CLUSTAL W(1.81) multiple sequence alignment 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ACGGCTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATC 481656_ENSG00000119866_HUMAN_9496_9947/1-454 ACGGCTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATC 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 -CGGCTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATC 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 -CAGCTCGGTTCACATCGGGAGAGCCGGGTTAGA---AAGGAGACTCCAGAGAAAATATC * ******************************* *********************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 TTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 TTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGA 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 TTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 TTCATCAATGCC-TTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGA ******* **** *********************************************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AGATGAATTGTGGGAGAGCCGTCATGGC----------NNNNNNNNNNNNNNAAGAAAAA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 AGATGAATTGTGGGAGAGCCGTCATGGCTTTTTTTTAAGCNNNNNNNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AGATGAATTGTGGGAGAGCCGTCATGGC---------------NNNNNNNNNNNNAAGGA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 AGATGGATTGTGGGATAGCCGTCATGGC---------------TTTTTTGAAAAGAGAGA ***** ********* ************ 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAA 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AAAA------AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GAGA------------------------------------------------GAGATG-A ** * 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AAAAATGGCAAAAG 481656_ENSG00000119866_HUMAN_9496_9947/1-454 AAAAATGGCAAAAG 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AAAAATGGCAAAAG 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 AAAAATGGCAAAAG **************
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 254 Mean pairwise identity: 78.07 Mean single sequence MFE: -45.68 Consensus MFE: -37.89 Energy contribution: -40.21 Covariance contribution: 2.31 Mean z-score: -2.41 Structure conservation index: 0.83 SVM decision value: 2.62 SVM RNA-class probability: 0.995865 Prediction: RNA ###################################################################### >481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ACGGCUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNNNAAGAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAG (((((((..((((((((.((((..((.............))...)))).)).....(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))...)))))).)))))))...................................................................................................... ( -44.82) >481656_ENSG00000119866_HUMAN_9496_9947/1-454 ACGGCUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCUUUUUUUUAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAG ..(((((..((((((((.((((..((.............))...)))).)).....(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))...)))))).)))))(((((.((((......))))............................................................................(....)..)))))..... ( -47.12) >481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CGGCUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNAAGGAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAG .(((((..((((((((.((((..((.............))...)))).)).....(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))...)))))).)))))(((((..............................................................................)))))..... ( -44.50) >481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CAGCUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGGAGACUCCAGAGAAAAUAUCUUCAUCAAUGCCUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGGAUUGUGGGAUAGCCGUCAUGGCUUUUUUGAAAAGAGAGAGAGAGAGAUGAAAAAAUGGCAAAAG .((((((((((.(.....).))))))))))....((....))(((.((....(((((((.((..((((((((.....))))....((((....))))........))))..)))))))))..)).)))....((((((((..(((((((.........)))))))..)))).....))))..... ( -46.30) >consensus _CGGCUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGC___________________________AAAGAAAAA____________________________________________________UGAAAAAAAUGGCAAAAG ..((((((((((.(.....).))))))))))........((...((((...((...(((((((.((.(((((.((((.....))))....((((....))))........))))).)))))))))..))...))))..))(((((...................................................................................................)))))..... (-37.89 = -40.21 + 2.31)
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