CLUSTAL W(1.81) multiple sequence alignment 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 CTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATCTTCA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 CTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATCTTCA 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CTCGGTTCACATCGGGAGAGCCGGGTTAGAAAGAAGGAGACTCCAGAGAAAATATCTTCA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CTCGGTTCACATCGGGAGAGCCGGGTTAGA---AAGGAGACTCCAGAGAAAATATCTTCA ****************************** *************************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 TCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGAAGAT 481656_ENSG00000119866_HUMAN_9496_9947/1-454 TCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGAAGAT 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 TCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGAAGAT 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 TCAATGCC-TTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCGCAGGGAAGAT *** **** *************************************************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 GAATTGTGGGAGAGCCGTCATGGC----------NNNNNNNNNNNNNNAAGAAAAAAAAA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 GAATTGTGGGAGAGCCGTCATGGCTTTTTTTTAAGCNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 GAATTGTGGGAGAGCCGTCATGGC---------------NNNNNNNNNNNNAAGGAAAAA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GGATTGTGGGATAGCCGTCATGGC---------------TTTTTTGAAAAGAGAGAGAGA * ********* ************ 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAAAA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAAAA 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 ------AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTGAAAAAA 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ------------------------------------------------GAGATG-AAAAA ** ***** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCCTGCGAACTTG 481656_ENSG00000119866_HUMAN_9496_9947/1-454 ATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCCTGCGAACTTG 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 ATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCCTGCGAACTTG 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCCTGCGAACTTG ************************************************************
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 81.92 Mean single sequence MFE: -62.20 Consensus MFE: -54.78 Energy contribution: -57.02 Covariance contribution: 2.25 Mean z-score: -1.28 Structure conservation index: 0.88 SVM decision value: -0.01 SVM RNA-class probability: 0.528877 Prediction: RNA ###################################################################### >481656_ENSRNOG00000007049_RAT_9511_9952/1-454 CUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNNNAAGAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUG ((((((((.(.....).)))))))).......(.((....)).).(((....(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))..(((..((.((..(((((((..........................................................................................(((....)))(((((((((.....)))).)).).)).)))))))))))..))).))). ( -60.80) >481656_ENSG00000119866_HUMAN_9496_9947/1-454 CUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCUUUUUUUUAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUG ((((((....))))))..(((((((((((((((((.....((((...((...(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))..))...)))).(((.....)))))))))))))........................................................................................(((....)))..)))).))).(((((((..(((.(((((...))))).)))))))))) ( -63.40) >481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNAAGGAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUG ((((((((.(.....).)))))))).......(.((....)).).(((....(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))..(((..((.((..(((((((...............................................................................(((....)))(((((((((.....)))).)).).)).)))))))))))..))).))). ( -60.80) >481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGGAGACUCCAGAGAAAAUAUCUUCAUCAAUGCCUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGGAUUGUGGGAUAGCCGUCAUGGCUUUUUUGAAAAGAGAGAGAGAGAGAUGAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUG ..(((((((....(((...(((((((((...((....))(((.((....(((((((.((..((((((((.....))))....((((....))))........))))..)))))))))..)).)))..))))).((((..(((((((.........)))))))..)))).....)))).....)))...)))))))(((((((..(((.(((((...))))).)))))))))) ( -63.80) >consensus CUCGGUUCACAUCGGGAGAGCCGGGUUAGAAAGAAGGAGACUCCAGAGAAAAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGC___________________________AAAGAAAAA____________________________________________________UGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUG ((((((((.(.....).)))))))).........((....))...(((....(((((((.((.(((((.((((.....))))....((((....))))........))))).)))))))))..((((((((....(((((((....................................................................................................(((....)))(((((((((.....)))).)).).)).))))))))))).)))).))). (-54.78 = -57.02 + 2.25)
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