CLUSTAL W(1.81) multiple sequence alignment 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AATATCTTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCG 481656_ENSG00000119866_HUMAN_9496_9947/1-454 AATATCTTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCG 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AATATCTTCATCAGTGCCTTTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCG 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 AATATCTTCATCAATGCC-TTTGACATCCAAAATAAATTAGAAATAATACAAAGATGGCG ************* **** ***************************************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 CAGGGAAGATGAATTGTGGGAGAGCCGTCATGGC----------NNNNNNNNNNNNNNAA 481656_ENSG00000119866_HUMAN_9496_9947/1-454 CAGGGAAGATGAATTGTGGGAGAGCCGTCATGGCTTTTTTTTAAGCNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CAGGGAAGATGAATTGTGGGAGAGCCGTCATGGC---------------NNNNNNNNNNN 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CAGGGAAGATGGATTGTGGGATAGCCGTCATGGC---------------TTTTTTGAAAA *********** ********* ************ 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 GAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 NAAGGAAAAA------AANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GAGAGAGAGA------------------------------------------------GA 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 NNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCC 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCC 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 NNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCC 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GATG-AAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTCAAGTGCGGACGTGACGTCCC ** ******************************************************* 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 TGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACACACAAAACATGGGCAGGGC-G 481656_ENSG00000119866_HUMAN_9496_9947/1-454 TGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACACACAAAACATGGGCAGGGC-G 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 TGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACACACAAAACATGGGCAGGGC-G 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 TGCGAACTTGAACGTCAGGAG-ATGGATGGACACAGACATACAGAACATGGGCAGGGCAA ********************* ********** ***** *** **************
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 81.21 Mean single sequence MFE: -62.80 Consensus MFE: -54.06 Energy contribution: -56.80 Covariance contribution: 2.75 Mean z-score: -1.82 Structure conservation index: 0.86 SVM decision value: 1.00 SVM RNA-class probability: 0.897705 Prediction: RNA ###################################################################### >481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNNNAAGAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCG ..(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))............((((((((.........................................................................................)))))....((((.(((((....(((((((..(((.(((((...))))).))))))))))....)))))..((((......))))..............))))..))). ( -61.14) >481656_ENSG00000119866_HUMAN_9496_9947/1-454 AAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCUUUUUUUUAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCG ..(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))..(((..((((((((.....))))))))..))).........................................................................................((....((((.(((((....(((((((..(((.(((((...))))).))))))))))....)))))..((((......))))..............))))...)). ( -65.10) >481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGCNNNNNNNNNNNNAAGGAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCG ..(((((((.((.((((((((((.....)))))...((((....))))........))))).)))))))))............((((((((..............................................................................)))))....((((.(((((....(((((((..(((.(((((...))))).))))))))))....)))))..((((......))))..............))))..))). ( -61.28) >481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 AAUAUCUUCAUCAAUGCCUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGGAUUGUGGGAUAGCCGUCAUGGCUUUUUUGAAAAGAGAGAGAGAGAGAUGAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGAUGGAUGGACACAGACAUACAGAACAUGGGCAGGGCAA ..(((((((.((..((((((((.....))))....((((....))))........))))..)))))))))............((((((((..(((((((.........)))))))..)))).....))))....(((((((((..(((...(((((((((((((((...)))))..))..))))))))........(((..((....))..))).)))..)).)))..)))).. ( -63.70) >consensus AAUAUCUUCAUCAGUGCCUUUUGACAUCCAAAAUAAAUUAGAAAUAAUACAAAGAUGGCGCAGGGAAGAUGAAUUGUGGGAGAGCCGUCAUGGC___________________________AAAGAAAAA____________________________________________________UGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGC_G ..(((((((.((.(((((.((((.....))))....((((....))))........))))).)))))))))............((((((((...................................................................................................)))))....((((.(((((....(((((((..(((.(((((...))))).))))))))))....)))))..((((......))))..............))))..))).. (-54.06 = -56.80 + 2.75)
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