CLUSTAL W(1.81) multiple sequence alignment 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ACTGGCCATTGGCTTGTCCTGGTCTCTTTTTCATGTCCAGCCCCTGATGTGTGTCCATTG 481656_ENSG00000119866_HUMAN_9496_9947/1-454 ACTGGCCATTGGCTTGTCCTGGTCTCTTTTTCATGTCCAGCCCCTGATGTGTGTCCATTG 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 -CTGGCCATTGGCTTGTCCTGGTCTCTTTTTCATGTCCAGCCCCTGATGTGTGTCCATTG 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 -CTGTCCATTGGCTTCTTCGGGTCTATTTTTCATGTCCAGCCCCTAATGAGTGTCCATTG 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 GTGT-GAGCTTCCCCTTCCCTCCTCCCCTTCTCTCCTGCTC-GCCCTGCCCATGTTTTGT 481656_ENSG00000119866_HUMAN_9496_9947/1-454 GTGT-GAGCTTCCCCTTCCCTCCTCCCCTTCTCTCCTGCTC-GCCCTGCCCATGTTTTGT 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 GTGT-GAGCTTCCCCTTCCCTCCTCCCCTTCTCTCCTGCTC-GCCCTGCCCATGTTTTGT 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GTTTTGTTATTTCCACTCCCTCCTCC--TACTCCACGGCTTTGCCCTGCCCATGTTCTGT 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 GTGTCTCTGTCCATCCAGACTCCTGACGTTCAAGTTCGCAGGGACGTCACGTCCGCACTT 481656_ENSG00000119866_HUMAN_9496_9947/1-454 GTGTCTCTGTCCATCCAGACTCCTGACGTTCAAGTTCGCAGGGACGTCACGTCCGCACTT 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 GTGTCTCTGTCCATCCAGACTCCTGACGTTCAAGTTCGCAGGGACGTCACGTCCGCACTT 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ATGTCTGTGTCCATCCAT-CTCCTGACGTTCAAGTTCGCAGGGACGTCACGTCCGCACTT 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 GAACTTGCAGCTCAGGGGGGCTTTTGCCATTTTTTTCANNNNNNNNNNNNNNNNNNNNNN 481656_ENSG00000119866_HUMAN_9496_9947/1-454 GAACTTGCAGCTCAGGGGGGCTTTTGCCATTTTTTTCANNNNNNNNNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 GAACTTGCAGCTCAGGGGGGCTTTTGCCATTTTTTTCANNNNNNNNNNNNNNNNNNNNNN 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 GAACTTGCAGCTCAGGGGGGCTTTTGCCATTTTTT-CATCTC------------------ 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTTTTTTTTTCTTNNNNNNNNNNNNNN---- 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCTTAA 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 NNNNNNNNNNNNNNNNNNNNNNTT------TTTTTCCTTNNNNNNNNNNNN--------- 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ------------------------------TCTCTCTCTCTTTTCAAAAAA---------
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 79.27 Mean single sequence MFE: -60.37 Consensus MFE: -50.72 Energy contribution: -52.09 Covariance contribution: 1.38 Mean z-score: -2.82 Structure conservation index: 0.84 SVM decision value: 3.19 SVM RNA-class probability: 0.998706 Prediction: RNA ###################################################################### >481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ACUGGCCAUUGGCUUGUCCUGGUCUCUUUUUCAUGUCCAGCCCCUGAUGUGUGUCCAUUGGUGUGAGCUUCCCCUUCCCUCCUCCCCUUCUCUCCUGCUCGCCCUGCCCAUGUUUUGUGUGUCUCUGUCCAUCCAGACUCCUGACGUUCAAGUUCGCAGGGACGUCACGUCCGCACUUGAACUUGCAGCUCAGGGGGGCUUUUGCCAUUUUUUUCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUUUUUUUUUCUUNNNNNNNNNNNNNN ..((((.(..((((....((((.(..........).))))(((((((.(((((..((..((.((((((............................))))))))))..)))......((((((...(((......)))....)))((((((((..((..(((((...)))))))))))))))..))))))))))))))))..)))))....................................................................................... ( -62.59) >481656_ENSG00000119866_HUMAN_9496_9947/1-454 ACUGGCCAUUGGCUUGUCCUGGUCUCUUUUUCAUGUCCAGCCCCUGAUGUGUGUCCAUUGGUGUGAGCUUCCCCUUCCCUCCUCCCCUUCUCUCCUGCUCGCCCUGCCCAUGUUUUGUGUGUCUCUGUCCAUCCAGACUCCUGACGUUCAAGUUCGCAGGGACGUCACGUCCGCACUUGAACUUGCAGCUCAGGGGGGCUUUUGCCAUUUUUUUCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGCUUAA ..((((.(..((((....((((.(..........).))))(((((((.(((((..((..((.((((((............................))))))))))..)))......((((((...(((......)))....)))((((((((..((..(((((...)))))))))))))))..))))))))))))))))..)))))........................................................................................... ( -62.59) >481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CUGGCCAUUGGCUUGUCCUGGUCUCUUUUUCAUGUCCAGCCCCUGAUGUGUGUCCAUUGGUGUGAGCUUCCCCUUCCCUCCUCCCCUUCUCUCCUGCUCGCCCUGCCCAUGUUUUGUGUGUCUCUGUCCAUCCAGACUCCUGACGUUCAAGUUCGCAGGGACGUCACGUCCGCACUUGAACUUGCAGCUCAGGGGGGCUUUUGCCAUUUUUUUCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUUUUUUUCCUUNNNNNNNNNNNN .((((.(..((((....((((.(..........).))))(((((((.(((((..((..((.((((((............................))))))))))..)))......((((((...(((......)))....)))((((((((..((..(((((...)))))))))))))))..))))))))))))))))..)))))............................................................................ ( -62.59) >481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CUGUCCAUUGGCUUCUUCGGGUCUAUUUUUCAUGUCCAGCCCCUAAUGAGUGUCCAUUGGUUUUGUUAUUUCCACUCCCUCCUCCUACUCCACGGCUUUGCCCUGCCCAUGUUCUGUAUGUCUGUGUCCAUCCAUCUCCUGACGUUCAAGUUCGCAGGGACGUCACGUCCGCACUUGAACUUGCAGCUCAGGGGGGCUUUUGCCAUUUUUUCAUCUCUCUCUCUCUCUUUUCAAAAAA ........((((.......((.(..........).))((((((..(((..((..(((.((.................................(((........)))((((.....)))))).)))..))..)))..(((((.(((((((((((..((((((...))))).)...)))))))).))))))))))))))...))))................................. ( -53.70) >consensus _CUGGCCAUUGGCUUGUCCUGGUCUCUUUUUCAUGUCCAGCCCCUGAUGUGUGUCCAUUGGUGU_GAGCUUCCCCUUCCCUCCUCCCCUUCUCUCCUGCUC_GCCCUGCCCAUGUUUUGUGUGUCUCUGUCCAUCCAGACUCCUGACGUUCAAGUUCGCAGGGACGUCACGUCCGCACUUGAACUUGCAGCUCAGGGGGGCUUUUGCCAUUUUUUUCA____________________________________________________UUUUUCUUU_____________________ .........((((.......((.(..........).))((((((.((((..((..(((.((((((((((............................)))))))...))).)))...))..))))((((......))))..(((((.(((((((((((..((((((...))))).)...)))))))).))))))))))))))...))))........................................................................................... (-50.72 = -52.09 + 1.38)
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