CLUSTAL W(1.81) multiple sequence alignment 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ----NNNNNNNNNNNNNNAAGAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSG00000119866_HUMAN_9496_9947/1-454 TTAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 ---------NNNNNNNNNNNNAAGGAAAAA------AANNNNNNNNNNNNNNNNNNNNNN 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ---------TTTTTTGAAAAGAGAGAGAGA------------------------------ 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 NNNNNNNNNNNNNNNNNNNNNNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTC 481656_ENSG00000119866_HUMAN_9496_9947/1-454 NNNNNNNNNNNNNNNNNNNNNNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTC 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 NNNNNNNNNNNNNNNNNNNNNNTGAAAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTC 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ------------------GAGATG-AAAAAATGGCAAAAGCCCCCCTGAGCTGCAAGTTC ** *********************************** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 AAGTGCGGACGTGACGTCCCTGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACAC 481656_ENSG00000119866_HUMAN_9496_9947/1-454 AAGTGCGGACGTGACGTCCCTGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACAC 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 AAGTGCGGACGTGACGTCCCTGCGAACTTGAACGTCAGGAGTCTGGATGGACAGAGACAC 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 AAGTGCGGACGTGACGTCCCTGCGAACTTGAACGTCAGGAG-ATGGATGGACACAGACAT ***************************************** ********** ***** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 ACAAAACATGGGCAGGGC-GAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCTC-ACAC 481656_ENSG00000119866_HUMAN_9496_9947/1-454 ACAAAACATGGGCAGGGC-GAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCTC-ACAC 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 ACAAAACATGGGCAGGGC-GAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCTC-ACAC 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 ACAGAACATGGGCAGGGCAAAGCCGTGGAGTA--GGAGGAGGGAGTGGAAATAACAAAAC *** ************** *** * *** * ********** ** ** * * ** 481656_ENSRNOG00000007049_RAT_9511_9952/1-454 CAATGGACACACATCAGGGGCTGGACATGAAAAAGAGACCAGGACAAGCCAATGGCCAGT 481656_ENSG00000119866_HUMAN_9496_9947/1-454 CAATGGACACACATCAGGGGCTGGACATGAAAAAGAGACCAGGACAAGCCAATGGCCAGT 481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 CAATGGACACACATCAGGGGCTGGACATGAAAAAGAGACCAGGACAAGCCAATGGCCAG- 481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 CAATGGACACTCATTAGGGGCTGGACATGAAAAATAGACCCGAAGAAGCCAATGGACAG- ********** *** ******************* ***** * * ********** ***
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 79.27 Mean single sequence MFE: -62.25 Consensus MFE: -50.20 Energy contribution: -51.51 Covariance contribution: 1.31 Mean z-score: -2.14 Structure conservation index: 0.81 SVM decision value: 1.74 SVM RNA-class probability: 0.974945 Prediction: RNA ###################################################################### >481656_ENSRNOG00000007049_RAT_9511_9952/1-454 NNNNNNNNNNNNNNAAGAAAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCGAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCUCACACCAAUGGACACACAUCAGGGGCUGGACAUGAAAAAGAGACCAGGACAAGCCAAUGGCCAGU .......................................................................................((((.....(((((((...((((..((((......))))....((.((((((...((((.....))))..((((......))))................)))))))).))))......))))).)).((...(((....)))...)).............((..(((((..(.((..........)))..).))))..)))))).. ( -62.50) >481656_ENSG00000119866_HUMAN_9496_9947/1-454 UUAAGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCGAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCUCACACCAAUGGACACACAUCAGGGGCUGGACAUGAAAAAGAGACCAGGACAAGCCAAUGGCCAGU ...........................................................................................((((.....(((((((...((((..((((......))))....((.((((((...((((.....))))..((((......))))................)))))))).))))......))))).)).((...(((....)))...)).............((..(((((..(.((..........)))..).))))..)))))).. ( -62.50) >481656_ENSMUSG00000000861_MOUSE_2861_3289/1-454 NNNNNNNNNNNNAAGGAAAAAAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGCGAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCUCACACCAAUGGACACACAUCAGGGGCUGGACAUGAAAAAGAGACCAGGACAAGCCAAUGGCCAG ............................................................................((((.....(((((((...((((..((((......))))....((.((((((...((((.....))))..((((......))))................)))))))).))))......))))).)).((...(((....)))...)).............((..(((((..(.((..........)))..).))))..)))))). ( -62.50) >481656_ENSDARG00000018362_ZEBRAFISH_9467_9847/1-454 UUUUUUGAAAAGAGAGAGAGAGAGAUGAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGAUGGAUGGACACAGACAUACAGAACAUGGGCAGGGCAAAGCCGUGGAGUAGGAGGAGGGAGUGGAAAUAACAAAACCAAUGGACACUCAUUAGGGGCUGGACAUGAAAAAUAGACCCGAAGAAGCCAAUGGACAG ((((((....)))))).................((((...((((((((((..(..(..(((((((((((((((...)))))..))..))))))))(((.....(((..((....))..))).((.....)))))..(((...))))..)..))))......(((((....................)))))....))))))((...............))......))))........ ( -61.51) >consensus _____________________AAAGAAAAA____________________________________________________UGAAAAAAAUGGCAAAAGCCCCCCUGAGCUGCAAGUUCAAGUGCGGACGUGACGUCCCUGCGAACUUGAACGUCAGGAGUCUGGAUGGACAGAGACACACAAAACAUGGGCAGGGC_GAGCAGGAGAGAAGGGGAGGAGGGAAGGGGAAGCUC_ACACCAAUGGACACACAUCAGGGGCUGGACAUGAAAAAGAGACCAGGACAAGCCAAUGGCCAG_ ...........................................................................................((((.......(((((((.((((..(((((((((((((((...)))))..))..))))))))(((.....((((......))))))).............)))).........................((...(((....)))....))............)))))))((((..............)))).....))))......... (-50.20 = -51.51 + 1.31)
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