CLUSTAL W(1.81) multiple sequence alignment 478948_ENSMUSG00000026565_MOUSE_4444_4538/1-97 -TGTCCGAGGAAGGCCTGGGCCTGGGCTGTGGAGATCGCTCCTCCAACGGGCACGGGCTG 478948_SINFRUG00000130458_FUGU_7759_7854/1-97 -TGTTGGAGGAGGGCTTGTGCATGTGCATTAGTGATTGCACTTGTGGTTGGCACCATTTG 478948_ENSRNOG00000003581_RAT_3735_3829/1-97 -TGCCCAAGGAAAGCCTGGGCCTGGGCTGTGGAGATGGCCCCTCCGACAGGCACGGGCTG 478948_ENSDARG00000007996_ZEBRAFISH_8031_8126/1-97 CTGTTGGAGCAAGGCCTGTGCGTGTGCGTTGGTGATTGCGCTTGTTGTTTGCACAGTCTG ** ** * ** ** ** ** ** * * *** ** * * **** ** 478948_ENSMUSG00000026565_MOUSE_4444_4538/1-97 TTTCTGAAAGTCCAGACCATTGGTTTGTGTGCCTGG- 478948_SINFRUG00000130458_FUGU_7759_7854/1-97 CCTCTGGAAATCCAATCCATTTATTTGGATACCTGGG 478948_ENSRNOG00000003581_RAT_3735_3829/1-97 CTTCTCAAAGTCCAGGCCATTGGTCTGTGTGCCTGG- 478948_ENSDARG00000007996_ZEBRAFISH_8031_8126/1-97 CCTCTGAAAGTCCAGTCCATTTGTTTGGGCACCTGG- *** ** **** ***** * ** *****
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 97 Mean pairwise identity: 70.83 Mean single sequence MFE: -36.17 Consensus MFE: -23.59 Energy contribution: -24.78 Covariance contribution: 1.19 Mean z-score: -1.33 Structure conservation index: 0.65 SVM decision value: 0.17 SVM RNA-class probability: 0.618566 Prediction: RNA ###################################################################### >478948_ENSMUSG00000026565_MOUSE_4444_4538/1-97 UGUCCGAGGAAGGCCUGGGCCUGGGCUGUGGAGAUCGCUCCUCCAACGGGCACGGGCUGUUUCUGAAAGUCCAGACCAUUGGUUUGUGUGCCUGG .((((.....((((....))))))))..(((((.......))))).((((((((((((.........)))))(((((...)))))..))))))). ( -38.10) >478948_SINFRUG00000130458_FUGU_7759_7854/1-97 UGUUGGAGGAGGGCUUGUGCAUGUGCAUUAGUGAUUGCACUUGUGGUUGGCACCAUUUGCCUCUGGAAAUCCAAUCCAUUUAUUUGGAUACCUGGG ..(..((((.(((((..((((.(((((........))))).))))...))).)).....))))..)....((((((((......)))))...))). ( -29.90) >478948_ENSRNOG00000003581_RAT_3735_3829/1-97 UGCCCAAGGAAAGCCUGGGCCUGGGCUGUGGAGAUGGCCCCUCCGACAGGCACGGGCUGCUUCUCAAAGUCCAGGCCAUUGGUCUGUGUGCCUGG ...(((.((..((((..((((((((((...((((.(((((..((....))...)))))...))))..))))))))))...)).)).....))))) ( -45.60) >478948_ENSDARG00000007996_ZEBRAFISH_8031_8126/1-97 CUGUUGGAGCAAGGCCUGUGCGUGUGCGUUGGUGAUUGCGCUUGUUGUUUGCACAGUCUGCCUCUGAAAGUCCAGUCCAUUUGUUUGGGCACCUGG ....((((.(((((((((((((...(((..((((....))))...))).)))))))...)))).))....))))(((((......)))))...... ( -31.10) >consensus _UGUCCGAGGAAGGCCUGGGCCUGGGCUGUGGAGAUUGCACCUCCGACUGGCACGGGCUGCCUCUGAAAGUCCAGUCCAUUGGUUUGGGUACCUGG_ ...(((((((..((((((((((((......((((....)))).....)))))))))))).)))))))..((((((((.....))))))))....... (-23.59 = -24.78 + 1.19)
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