CLUSTAL W(1.81) multiple sequence alignment 469885_ENSG00000169840_HUMAN_9566_9739/1-175 A-GGGAGAGGAGGGGGCGAGGGCGCCTGTTACGGCGTGGGTGGGGTTGACAAGAATAGAG 469885_SINFRUG00000149945_FUGU_9675_9844/1-175 A-AAGAGAGAATGGGCAGTGC-CGCCTCTTGCGGCGTGGGTGGGGCTGACAAGAATAGAC 469885_ENSRNOG00000000952_RAT_9566_9739/1-175 -GGGAAGAGGAGGGGGCGAGTGCGCCTGTTACGGCGTGGGTGGGGTTGACAAGAATAGAG 469885_ENSDARG00000026734_ZEBRAFISH_9427_9598/1-175 A-AAGGGAGAAGGGGCAGTAC-CGCCTCTTGCAGTGTGGGTGGGGCTGACAAGAATAGAC *** * *** * ***** ** * * ********** ************* 469885_ENSG00000169840_HUMAN_9566_9739/1-175 TACATTATGCAGTTCATTTAGTTAACAAGTGAAATAATGAGGAAGCGTGCAGAGTGAATG 469885_SINFRUG00000149945_FUGU_9675_9844/1-175 TACATTATGCAGTTCATTTAGTTAACAAGTGAAATAATGGGGAAGCGTGCAGTGGGAATG 469885_ENSRNOG00000000952_RAT_9566_9739/1-175 TACATTATGCAGTTCATTTAGTTAACAAGTGAAATAATGAGGAAGCGTGCAGAGTGAATG 469885_ENSDARG00000026734_ZEBRAFISH_9427_9598/1-175 TACATTATGCAGTTCATTTAGTTAACAAGTGAAATAATAGGGAAGCGTGCAGAGTGAATG ************************************** ************ * ***** 469885_ENSG00000169840_HUMAN_9566_9739/1-175 CCAAGAGAAAAGGCACAAAAACCCTATTGAGAGGCCCCGGCCGCTCCTGGCGTAG 469885_SINFRUG00000149945_FUGU_9675_9844/1-175 CCGAGAGAAA-GGCACAAAA-CCCTATTGAGAGCTTTTGGCCGCTTACAGCGTA- 469885_ENSRNOG00000000952_RAT_9566_9739/1-175 CCAAGAGAAAGGGCACAAAAACCCTATTGAGAGGCCCCGGCCGCTCCTGGCGTAG 469885_ENSDARG00000026734_ZEBRAFISH_9427_9598/1-175 CCGAGAGAAAAGGCACAAAA-CCCTATTGAGAGCTCTCAGCCGCTAGTAGCGTAA ** ******* ********* ************ ****** ***** //
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 175 Mean pairwise identity: 86.41 Mean single sequence MFE: -52.27 Consensus MFE: -34.35 Energy contribution: -37.85 Covariance contribution: 3.50 Mean z-score: -1.55 Structure conservation index: 0.66 SVM decision value: -0.05 SVM RNA-class probability: 0.506078 Prediction: RNA ###################################################################### >469885_ENSG00000169840_HUMAN_9566_9739/1-175 AGGGAGAGGAGGGGGCGAGGGCGCCUGUUACGGCGUGGGUGGGGUUGACAAGAAUAGAGUACAUUAUGCAGUUCAUUUAGUUAACAAGUGAAAUAAUGAGGAAGCGUGCAGAGUGAAUGCCAAGAGAAAAGGCACAAAAACCCUAUUGAGAGGCCCCGGCCGCUCCUGGCGUAG .(((((.((..(((((....(((((......))))).(((((((((...............((((.((((.(((((((.(....)))))))).......(....).)))).))))..((((.........))))....))))))))).....)))))..)).)))))....... ( -58.10) >469885_SINFRUG00000149945_FUGU_9675_9844/1-175 AAAGAGAGAAUGGGCAGUGCCGCCUCUUGCGGCGUGGGUGGGGCUGACAAGAAUAGACUACAUUAUGCAGUUCAUUUAGUUAACAAGUGAAAUAAUGGGGAAGCGUGCAGUGGGAAUGCCGAGAGAAAGGCACAAAACCCUAUUGAGAGCUUUUGGCCGCUUACAGCGUA ..........((.((...(((((.....)))))((((((((...................((((((....(((((((.(....)))))))))))))).((((((...(((((((..((((........))))......)))))))...))))))..)))))))).)).)) ( -50.30) >469885_ENSRNOG00000000952_RAT_9566_9739/1-175 GGGAAGAGGAGGGGGCGAGUGCGCCUGUUACGGCGUGGGUGGGGUUGACAAGAAUAGAGUACAUUAUGCAGUUCAUUUAGUUAACAAGUGAAAUAAUGAGGAAGCGUGCAGAGUGAAUGCCAAGAGAAAGGGCACAAAAACCCUAUUGAGAGGCCCCGGCCGCUCCUGGCGUAG ((((.(.((..(((((....(((((......))))).(((((((((...............((((.((((.(((((((.(....)))))))).......(....).)))).))))..((((.........))))....))))))))).....)))))..)).)))))....... ( -53.70) >469885_ENSDARG00000026734_ZEBRAFISH_9427_9598/1-175 AAAGGGAGAAGGGGCAGUACCGCCUCUUGCAGUGUGGGUGGGGCUGACAAGAAUAGACUACAUUAUGCAGUUCAUUUAGUUAACAAGUGAAAUAAUAGGGAAGCGUGCAGAGUGAAUGCCGAGAGAAAAGGCACAAAACCCUAUUGAGAGCUCUCAGCCGCUAGUAGCGUAA ...((((((((((((......)))))))(((((((((...(..((....))..)...))))))..)))..(((((((.(....)))))))).((((((((..((.......))...((((.........)))).....))))))))....)))))...(((.....)))... ( -47.00) >consensus A_AAGAGAGAAGGGGCAGAGC_CGCCUCUUACGGCGUGGGUGGGGCUGACAAGAAUAGACUACAUUAUGCAGUUCAUUUAGUUAACAAGUGAAAUAAUGAGGAAGCGUGCAGAGUGAAUGCCAAGAGAAAAGGCACAAAA_CCCUAUUGAGAGCCCCCGGCCGCUCCUAGCGUAG .......(((((.(((((..((((((......))))))(((((((((...............((((.((((.(((((((.(....)))))))).......(....).)))).))))..((((.........))))....))))))))).........))))).)))))....... (-34.35 = -37.85 + 3.50)
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