CLUSTAL W(1.81) multiple sequence alignment 461739_ENSMUSG00000034255_MOUSE_8785_8894/1-112 -CCCTCCAGCTTCGAAGCCGAGATGGCTC-GGAGTACCTGATCCAGCACGACTCCGAAGC 461739_SINFRUG00000131017_FUGU_9484_9593/1-112 CCTCCCCAG-TTAAAAACTAAGAACGCGGTGGAATTCCTGATACAGTACGACACAGAAAG 461739_ENSRNOG00000028569_RAT_8775_8884/1-112 -CCCTCCAGCTCCGGAGCCGAGATGGCTC-AGAGTACCTGATCCAGCATGACTCAGAGGC 461739_ENSDARG00000006344_ZEBRAFISH_6913_7022/1-112 CTCATTCAGCTTAAGACTCGGACTGGTGC-CGAATACTTGATCCAGTACGACACCGATAG *** * * * ** * * **** *** * *** * ** 461739_ENSMUSG00000034255_MOUSE_8785_8894/1-112 CATCATCAGCACCTGGCACAAAGCCATTGCCGAAGGCATCTCAGAGCTGGTA 461739_SINFRUG00000131017_FUGU_9484_9593/1-112 CATCATTGTTGACTGGCACCGAGTCCTGACCGATACCATACGACAACTGGT- 461739_ENSRNOG00000028569_RAT_8775_8884/1-112 CATCATCAGCACCTGGCACAAGGCCATTGCCGAAGGCATCGAGGAGCTGGTA 461739_ENSDARG00000006344_ZEBRAFISH_6913_7022/1-112 CATCATCCAAGACTGGCATAAAGTTATCTTAGATACCATACACCAGCTGGT- ****** ****** * * ** *** * ***** //
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 112 Mean pairwise identity: 65.37 Mean single sequence MFE: -36.25 Consensus MFE: -16.50 Energy contribution: -18.75 Covariance contribution: 2.25 Mean z-score: -2.29 Structure conservation index: 0.46 SVM decision value: 1.60 SVM RNA-class probability: 0.966849 Prediction: RNA ###################################################################### >461739_ENSMUSG00000034255_MOUSE_8785_8894/1-112 CCCUCCAGCUUCGAAGCCGAGAUGGCUCGGAGUACCUGAUCCAGCACGACUCCGAAGCCAUCAUCAGCACCUGGCACAAAGCCAUUGCCGAAGGCAUCUCAGAGCUGGUA ....((((((..((.(((..(((((((((((((..(((...)))....)))))).))))))).((.(((..((((.....)))).))).)).)))...))..)))))).. ( -49.40) >461739_SINFRUG00000131017_FUGU_9484_9593/1-112 CCUCCCCAGUUAAAAACUAAGAACGCGGUGGAAUUCCUGAUACAGUACGACACAGAAAGCAUCAUUGUUGACUGGCACCGAGUCCUGACCGAUACCAUACGACAACUGGU .....((((((........((.((.((((((....)).....((((.(((((..((.....))..)))))))))..)))).)).))...((........))..)))))). ( -23.90) >461739_ENSRNOG00000028569_RAT_8775_8884/1-112 CCCUCCAGCUCCGGAGCCGAGAUGGCUCAGAGUACCUGAUCCAGCAUGACUCAGAGGCCAUCAUCAGCACCUGGCACAAGGCCAUUGCCGAAGGCAUCGAGGAGCUGGUA ....((((((((.(((((..((((((((.((((..(((...)))....)))).)).)))))).((.(((..((((.....)))).))).)).))).))..)))))))).. ( -48.70) >461739_ENSDARG00000006344_ZEBRAFISH_6913_7022/1-112 CUCAUUCAGCUUAAGACUCGGACUGGUGCCGAAUACUUGAUCCAGUACGACACCGAUAGCAUCAUCCAAGACUGGCAUAAAGUUAUCUUAGAUACCAUACACCAGCUGGU .....((((((......(((.(((((...(((....)))..))))).)))....((((((...(((((....))).))...))))))................)))))). ( -23.00) >consensus _CCCUCCAGCUUAAAAGCCGAGAUGGCGC_GGAAUACCUGAUCCAGCACGACACAGAAACCAUCAUCAGCAACUGGCACAAAGCCAUUGCCGAAACCAUACAACAGCUGGU_ .....(((((((.........(((((((...((((..(((...)))....))))...))))))).((.((((.((((.....)))))))).))..........))))))).. (-16.50 = -18.75 + 2.25)
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