CLUSTAL W(1.81) multiple sequence alignment 461722_ENSMUSG00000034255_MOUSE_6286_6370/1-86 CACCTCTAACACATTCTTCTTGCTGGATTTGTCTTTGGGGGCCCAAGAGAGAGAGGCCCC 461722_ENSDARG00000006344_ZEBRAFISH_5690_5774/1-86 -ACCTCCAGAACGTTTTTCTTGCTTGATTTGTCCTTGGGCGCCCGGTTAATTGTGGCGCC 461722_SINFRUG00000131017_FUGU_9289_9374/1-86 AACCTCCAAAACATTCTTTTTGCTGGATTTGTCTTTTGTGGCCCAGCAAATAGTCGCTCC 461722_ENSRNOG00000028569_RAT_5899_5983/1-86 CACCTCTAGCACATTCTTCTTGCTGGATTTGTCTTTGGGGGCCCACGAGAGCGAGGCCCC ***** * ** ** ** ***** ******** ** * **** * * ** ** 461722_ENSMUSG00000034255_MOUSE_6286_6370/1-86 CTTCAGTTCGACTGTGTATTCAGGG- 461722_ENSDARG00000006344_ZEBRAFISH_5690_5774/1-86 TTTCAGATCCACTGTGAATTCTGGGA 461722_SINFRUG00000131017_FUGU_9289_9374/1-86 TTTCAGGTCCACTGTGACCTCTGGGA 461722_ENSRNOG00000028569_RAT_5899_5983/1-86 CCTCAGTTCGACTGTATATTCAGGG- **** ** ***** ** ***
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 86 Mean pairwise identity: 76.89 Mean single sequence MFE: -26.90 Consensus MFE: -14.71 Energy contribution: -15.78 Covariance contribution: 1.06 Mean z-score: -2.03 Structure conservation index: 0.55 SVM decision value: 0.38 SVM RNA-class probability: 0.710848 Prediction: RNA ###################################################################### >461722_ENSMUSG00000034255_MOUSE_6286_6370/1-86 CACCUCUAACACAUUCUUCUUGCUGGAUUUGUCUUUGGGGGCCCAAGAGAGAGAGGCCCCCUUCAGUUCGACUGUGUAUUCAGGG ..(((...(((((.((.....((((((.........((((((((........).)))))))))))))..)).)))))....))). ( -28.20) >461722_ENSDARG00000006344_ZEBRAFISH_5690_5774/1-86 ACCUCCAGAACGUUUUUCUUGCUUGAUUUGUCCUUGGGCGCCCGGUUAAUUGUGGCGCCUUUCAGAUCCACUGUGAAUUCUGGGA ...(((((((.....(((......((((((.....((((((((((....))).)))))))..))))))......)))))))))). ( -27.50) >461722_SINFRUG00000131017_FUGU_9289_9374/1-86 AACCUCCAAAACAUUCUUUUUGCUGGAUUUGUCUUUUGUGGCCCAGCAAAUAGUCGCUCCUUUCAGGUCCACUGUGACCUCUGGGA ...............((.((((((((....(((......))))))))))).))....((((...(((((......)))))..)))) ( -22.90) >461722_ENSRNOG00000028569_RAT_5899_5983/1-86 CACCUCUAGCACAUUCUUCUUGCUGGAUUUGUCUUUGGGGGCCCACGAGAGCGAGGCCCCCCUCAGUUCGACUGUAUAUUCAGGG ..(((((((((.........))))))).........((((((((.(....).).)))))))..(((.....)))........)). ( -29.00) >consensus CACCUCCAAAACAUUCUUCUUGCUGGAUUUGUCUUUGGGGGCCCAGGAAAGAGAGGCCCCCUUCAGUUCCACUGUGAAUUCAGGG_ ..((.....................((((((.....((((((((........).)))))))..))))))..(((......))))). (-14.71 = -15.78 + 1.06)
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