CLUSTAL W(1.81) multiple sequence alignment 414341_ENSG00000140416_HUMAN_5624_5696/1-73 GGCCTTTTTCTCTGCCAGCTCCAGCTTCTCCTGGGCATCTTTGAGAGCCTCAGAGTATTT 414341_ENSMUSG00000027940_MOUSE_4036_4106/1-73 -GCCTTCTTCTCTGCTAGCTCCAGCTTCTCCTGAGCATCCTTTAAAGCTTCCGAATACTT 414341_SINFRUG00000130484_FUGU_7389_7461/1-73 GGCTTTCTTTTCTGCCAGTTCAAGCTTCTCCTGAGCATCTTTAAGAGCCTCGGAGAACTT 414341_ENSRNOG00000018184_RAT_5891_5963/1-73 GGCCTTTTTCTCCGCCAGCTCCAGTTTCTCCTGGGCATCTTTGAGAGCCTCGGAGTATTT ** ** ** ** ** ** ** ** ******** ***** ** * *** ** ** * ** 414341_ENSG00000140416_HUMAN_5624_5696/1-73 GTCCAGTTCATCT 414341_ENSMUSG00000027940_MOUSE_4036_4106/1-73 GTCCAGCTCATC- 414341_SINFRUG00000130484_FUGU_7389_7461/1-73 GTCCAACTCATCT 414341_ENSRNOG00000018184_RAT_5891_5963/1-73 GTCCAGTTCATCT ***** *****
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 73 Mean pairwise identity: 83.79 Mean single sequence MFE: -17.12 Consensus MFE: -9.82 Energy contribution: -11.20 Covariance contribution: 1.38 Mean z-score: -2.21 Structure conservation index: 0.57 SVM decision value: 0.13 SVM RNA-class probability: 0.596319 Prediction: RNA ###################################################################### >414341_ENSG00000140416_HUMAN_5624_5696/1-73 GGCCUUUUUCUCUGCCAGCUCCAGCUUCUCCUGGGCAUCUUUGAGAGCCUCAGAGUAUUUGUCCAGUUCAUCU (((..........)))(((....)))....(((((((.(((((((...)))))))....)))))))....... ( -21.00) >414341_ENSMUSG00000027940_MOUSE_4036_4106/1-73 GCCUUCUUCUCUGCUAGCUCCAGCUUCUCCUGAGCAUCCUUUAAAGCUUCCGAAUACUUGUCCAGCUCAUC ................((((.((......)))))).........((((..(((....)))...)))).... ( -7.60) >414341_SINFRUG00000130484_FUGU_7389_7461/1-73 GGCUUUCUUUUCUGCCAGUUCAAGCUUCUCCUGAGCAUCUUUAAGAGCCUCGGAGAACUUGUCCAACUCAUCU (((..........))).(..((((.((((((.(((..((.....))..)))))))))))))..)......... ( -21.10) >414341_ENSRNOG00000018184_RAT_5891_5963/1-73 GGCCUUUUUCUCCGCCAGCUCCAGUUUCUCCUGGGCAUCUUUGAGAGCCUCGGAGUAUUUGUCCAGUUCAUCU (((..........)))..............(((((((.(((((((...)))))))....)))))))....... ( -18.80) >consensus GGCCUUCUUCUCUGCCAGCUCCAGCUUCUCCUGAGCAUCUUUGAGAGCCUCGGAGUACUUGUCCAGCUCAUCU (((..........)))(((....)))....(((((((.(((((((...)))))))....)))))))....... ( -9.82 = -11.20 + 1.38)
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