CLUSTAL W(1.81) multiple sequence alignment 414303_ENSG00000143549_HUMAN_1805_1947/1-143 TAGCTGGAGGATGAGCTGGCAGCCATGCAGAAGAAGCTGAAAGGGACAGAGGATGAGCTG 414303_SINFRUG00000130484_FUGU_7340_7481/1-143 -AGCTTGAGGACGATTTGGTAGCCCTGCAGAAGAAATTAAAGGGGACAGAAGATGAGTTG 414303_ENSMUSG00000027940_MOUSE_3986_4126/1-143 -AGCTAGAGGATGAACTAGCAACCATGCAGAAGAAGCTGAAAGGGACAGAGGATGAGCTG 414303_ENSRNOG00000017441_RAT_4521_4661/1-143 -AGCTAGAGGATGAACTAGCAACCATGCAGAAGAAGCTGAAAGGGACAGAGGATGAGCTG **** ***** ** * * * ** ********** * ** ******** ****** ** 414303_ENSG00000143549_HUMAN_1805_1947/1-143 GACAAGTATTCTGAAGCTTTGAAGGATGCCCAGGAGAAGCTGGAACTGGCAGAGAAGAAG 414303_SINFRUG00000130484_FUGU_7340_7481/1-143 GACAAGTTCTCCGAGGCTCTTAAAGATGCTCAGGAGAAGCTTGAACTGGCAGAAAAGAAA 414303_ENSMUSG00000027940_MOUSE_3986_4126/1-143 GACAAGTATTCGGAAGCTTTAAAGGATGCTCAGGAGAAGCTGGAGCTAGCAGAGAAGAAG 414303_ENSRNOG00000017441_RAT_4521_4661/1-143 GACAAGTATTCTGAGGCTTTAAAGGATGCCCAGGAGAAGCTGGAGCTAGCAGAGAAGAAG ******* ** ** *** * ** ***** *********** ** ** ***** ***** 414303_ENSG00000143549_HUMAN_1805_1947/1-143 GCTGCTGATGTGAGTATTAGAGA 414303_SINFRUG00000130484_FUGU_7340_7481/1-143 GCCACAGACGTAAGTATGGGGGA 414303_ENSMUSG00000027940_MOUSE_3986_4126/1-143 GCAGCCGACGTGAGTATTGGGG- 414303_ENSRNOG00000017441_RAT_4521_4661/1-143 GCAGCGGATGTGAGTATTGGGA- ** * ** ** ***** * //
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 143 Mean pairwise identity: 84.19 Mean single sequence MFE: -35.94 Consensus MFE: -24.88 Energy contribution: -24.50 Covariance contribution: -0.37 Mean z-score: -1.66 Structure conservation index: 0.69 SVM decision value: 0.21 SVM RNA-class probability: 0.638540 Prediction: RNA ###################################################################### >414303_ENSG00000143549_HUMAN_1805_1947/1-143 UAGCUGGAGGAUGAGCUGGCAGCCAUGCAGAAGAAGCUGAAAGGGACAGAGGAUGAGCUGGACAAGUAUUCUGAAGCUUUGAAGGAUGCCCAGGAGAAGCUGGAACUGGCAGAGAAGAAGGCUGCUGAUGUGAGUAUUAGAGA .((((........))))(((((((...(((((...(((.....(..(((........)))..).))).)))))...(((((..((.(..((((......)))).)))..))))).....)))))))................. ( -37.90) >414303_SINFRUG00000130484_FUGU_7340_7481/1-143 AGCUUGAGGACGAUUUGGUAGCCCUGCAGAAGAAAUUAAAGGGGACAGAAGAUGAGUUGGACAAGUUCUCCGAGGCUCUUAAAGAUGCUCAGGAGAAGCUUGAACUGGCAGAAAAGAAAGCCACAGACGUAAGUAUGGGGGA .((((........((((....((((....((....))...)))).))))......((..(.((((((((((..(((((.....)).)))..)))).))))))..)..))........))))..((.((....)).))..... ( -36.80) >414303_ENSMUSG00000027940_MOUSE_3986_4126/1-143 AGCUAGAGGAUGAACUAGCAACCAUGCAGAAGAAGCUGAAAGGGACAGAGGAUGAGCUGGACAAGUAUUCGGAAGCUUUAAAGGAUGCUCAGGAGAAGCUGGAGCUAGCAGAGAAGAAGGCAGCCGACGUGAGUAUUGGGG .(((((........)))))..((.....((((...(((((..(..(((........)))..).....)))))...))))....((((((((.(....((((....)))).........((...))..).)))))))))).. ( -34.00) >414303_ENSRNOG00000017441_RAT_4521_4661/1-143 AGCUAGAGGAUGAACUAGCAACCAUGCAGAAGAAGCUGAAAGGGACAGAGGAUGAGCUGGACAAGUAUUCUGAGGCUUUAAAGGAUGCCCAGGAGAAGCUGGAGCUAGCAGAGAAGAAGGCAGCGGAUGUGAGUAUUGGGA .(((((........)))))..((((((.......((((.......((.....)).(((((...(((.(((((.(((..........))))))))...)))....)))))...........))))........))).))).. ( -35.06) >consensus _AGCUAGAGGAUGAACUAGCAACCAUGCAGAAGAAGCUGAAAGGGACAGAGGAUGAGCUGGACAAGUAUUCUGAAGCUUUAAAGGAUGCCCAGGAGAAGCUGGAACUAGCAGAGAAGAAGGCAGCAGACGUGAGUAUUGGGG_ ..(((((........)))))..((((((.......(((.....(..(((........)))..).)))..((((.((((((......(((((((......)))).....)))......)))))).)))).....))).)))... (-24.88 = -24.50 + -0.37)
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