CLUSTAL W(1.81) multiple sequence alignment 376858_SINFRUG00000142471_FUGU_3228_3898/1-798 GTTGCTTCCCGGCAGC-AA-CAGATACTCACGTCATTCACTTGCTGTTCCAGTTTGGACT 376858_ENSRNOG00000002987_RAT_2681_3395/1-798 GTTTCAGGAGGGCATTTGA-AACATGCTCACATCATCCACTTGCTGTTCGAGCTTGGTTT 376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 AATTTACTTCCATGTTTAATGAAGAGCTCACATCATCAACTTGCTGCTCAAGCTTTGTCT 376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 ACTTCAGAAGGCCATCTGA-ACCGTGCTTACGTCATCCACTTGCTGTTCCAGCTTGGTTT . * : . .* . .:.** **.**** .******** ** ** ** *: * 376858_SINFRUG00000142471_FUGU_3228_3898/1-798 TGGCTTTGGTCAAGGTGTTGACCTTATCTTCCTCAGCCTGAAGGTCATCCAGAACCTGCT 376858_ENSRNOG00000002987_RAT_2681_3395/1-798 TGGCTTTGGTCAGGGTGTTGACTTTGTCCTCCTCTGCCTGCAGGTCATCCAGGGTCTGCT 376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 TGGATTTGGTCAGGGTGTTGACTTTGTCCTCCTCAGCCTGAAGATCATCCAGAGTCTGCT 376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 TGGCTTTGGTCAGGGTGTTGACTTTGTCCTCCTCTGCCTGCAGGTCATCCAGGGTCTGCT ***.********.********* **.** *****:*****.**.********.. ***** 376858_SINFRUG00000142471_FUGU_3228_3898/1-798 GATGGGCTTCCTGAAGGGCTCTCTTTTCCTTTGTCAGTTTGCCAATGTTCTCATCCTGAC 376858_ENSRNOG00000002987_RAT_2681_3395/1-798 GGTGGGCCTCCTGCAAGGCCTTCTTCTCCTTGGTCAGCTTGGCGATGGTTTCGTCCAGGC 376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 GATGTGCCTCTTGGAGGGCTTTCTTCTCCTTTGTAAGCTTGGCAATGCTCTCATCTTGAG 376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 GGTGGGCCTCCTGGAGGGCCTTCTTCTCCTTGGTCAGCTTGGCGATATTTTCATCCAGGC *.** ** ** ** *.*** **** ***** **.** *** *.**. * **.** :*. 376858_SINFRUG00000142471_FUGU_3228_3898/1-798 CACTCAGCTCGTCCATTAGATTTTTCA----TCTGC--AG-----ACAGACAC------- 376858_ENSRNOG00000002987_RAT_2681_3395/1-798 CCGCCATCTCCTCTGTGAGGTTTTTCACCT-TAGGA--AG----CAAACACACTTTG--- 376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 ATGCCATTTCCTCTGTCAAGTTCTTGACCTGTGTGATTTGATTTCAAATACACTTAGNNN 376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 CTGCCATCTCCTCTGTCAGGTTTTTCACCT-TGGGA--AG----CAAACGTGCTTTG--- . ** ** ** .* *..** ** * * *. :* *.* . .* 376858_SINFRUG00000142471_FUGU_3228_3898/1-798 ------------------------------------------------------------ 376858_ENSRNOG00000002987_RAT_2681_3395/1-798 --------------------------------------------------------CATT 376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 --------------------------------------------------------CATT
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 64.92 Mean single sequence MFE: -71.95 Consensus MFE: -33.39 Energy contribution: -34.45 Covariance contribution: 1.06 Mean z-score: -1.78 Structure conservation index: 0.46 SVM decision value: 0.54 SVM RNA-class probability: 0.776250 Prediction: RNA ###################################################################### >376858_SINFRUG00000142471_FUGU_3228_3898/1-798 GUUGCUUCCCGGCAGCAACAGAUACUCACGUCAUUCACUUGCUGUUCCAGUUUGGACUUGGCUUUGGUCAAGGUGUUGACCUUAUCUUCCUCAGCCUGAAGGUCAUCCAGAACCUGCUGAUGGGCUUCCUGAAGGGCUCUCUUUUCCUUUGUCAGUUUGCCAAUGUUCUCAUCCUGACCACUCAGCUCGUCCAUUAGAUUUUUCAUCUGCAGACAGACAC ((((((....))))))....(((......))).......(((((((.(((..(((((..((((.((((((.((((..(((........(.(((((....((((........))))))))).)(((...((((((((........))))...))))...)))...)))..)))).))))))...)))).)))))...((....))..)))..))))).)). ( -59.30) >376858_ENSRNOG00000002987_RAT_2681_3395/1-798 GUUUCAGGAGGGCAUUUGAAACAUGCUCACAUCAUCCACUUGCUGUUCGAGCUUGGUUUUGGCUUUGGUCAGGGUGUUGACUUUGUCCUCCUCUGCCUGCAGGUCAUCCAGGGUCUGCUGGUGGGCCUCCUGCAAGGCCUUCUUCUCCUUGGUCAGCUUGGCGAUGGUUUCGUCCAGGCCCGCCAUCUCCUCUGUGAGGUUUUUCACCUUAGGAAGCAAACACACUUUGCAUU .....(((((((((.(..((((..(((((((.((......)).)))..))))...))))..)....((((((....)))))).)))))))))..(((.((((..(......)..)))).)))((((((......)))))).....(((.((((..(((((((((.....))).))))))..)))).........((((((.....))))))))).(((((.....)))))... ( -82.60) >376858_ENSDARG00000014514_ZEBRAFISH_217_1012/1-798 AAUUUACUUCCAUGUUUAAUGAAGAGCUCACAUCAUCAACUUGCUGCUCAAGCUUUGUCUUGGAUUUGGUCAGGGUGUUGACUUUGUCCUCCUCAGCCUGAAGAUCAUCCAGAGUCUGCUGAUGUGCCUCUUGGAGGGCUUUCUUCUCCUUUGUAAGCUUGGCAAUGCUCUCAUCUUGAGAUGCCAUUUCCUCUGUCAAGUUCUUGACCUGUGUGAUUUGAUUUCAAAUACACUUAGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN ..........(((.....)))(((((..(((((((.(((((((((.....))).......(((((....(((((.((..((........))..)).))))).....))))))))).)).))))))).)))))(((((((((.(.........).)))).(((((....((((.....)))))))))...)))))(((((....)))))..(((((.((((....)))))))))................................................................... ( -66.00) >376858_ENSMUSG00000055724_MOUSE_2723_3438/1-798 ACUUCAGAAGGCCAUCUGAACCGUGCUUACGUCAUCCACUUGCUGUUCCAGCUUGGUUUUGGCUUUGGUCAGGGUGUUGACUUUGUCCUCCUCUGCCUGCAGGUCAUCCAGGGUCUGCUGGUGGGCCUCCUGGAGGGCCUUCUUCUCCUUGGUCAGCUUGGCGAUAUUUUCAUCCAGGCCUGCCAUCUCCUCUGUCAGGUUUUUCACCUUGGGAAGCAAACGUGCUUUGCAUU .......(((((((...((((((.(((.(((.((......)).)))...))).)))))))))))))..((((((((..(((((.(((((((((..((.((((..(......)..)))).))..))......)))))))...........((((..((((((.((.....))..))))))..))))...........)))))...))))))))((((((....))))))..... ( -79.90) >consensus AUUUCAGAACGGCAUCUAA_AACAUGCUCACAUCAUCCACUUGCUGUUCCAGCUUGGUCUUGGCUUUGGUCAGGGUGUUGACUUUGUCCUCCUCAGCCUGAAGGUCAUCCAGAGUCUGCUGAUGGGCCUCCUGGAGGGCCUUCUUCUCCUUGGUCAGCUUGGCAAUGUUCUCAUCCAGACCUGCCAUCUCCUCUGUCAGGUUUUUCACCU_UGGGA__AG____CAAACACACUUUG_______________________________________________________________ .........................(((.((.....(((...(((.....))).)))....(((...((.((((((....)))))).))......))).(((((((.(((((((...((......)).))))))).))))))).........)).))).(((((..((((.......))))))))).................................................................................................................. (-33.39 = -34.45 + 1.06)
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