CLUSTAL W(1.81) multiple sequence alignment 363120_ENSG00000104313_HUMAN_8178_8286/1-114 AAAGAGAAAAAGGAGGATACTGCAGCAGTGAAAGGCATATTGTCTTTAAGTT-GAGGTCT 363120_ENSDARG00000014259_ZEBRAFISH_7958_8067/1-114 -AAGGGGAAGA-GAGGAGTCGGTGGAACAGAGAGGCACATTGTCTGTAGGTTCAACCCCT 363120_ENSMUSG00000025932_MOUSE_8201_8311/1-114 AACGAGAAAAGGGAGGATACTGCAGCCAGGAAAGGCATAGTGTCTTTAAGTG-GAGGGCT 363120_ENSRNOG00000007590_RAT_8241_8351/1-114 AAAGAGAAAAGGGAGGATACTGCGGCCAGGAAAGGCATAGTATCTTTAAGTG-GAGGGCT * * * ** ***** * * * ** ***** * * *** ** ** * ** 363120_ENSG00000104313_HUMAN_8178_8286/1-114 CAACTGTTGTTTCCTCC-TGCAGGTCTATCCTCCT--CCTCCCCTTGTCAGGG- 363120_ENSDARG00000014259_ZEBRAFISH_7958_8067/1-114 CCACCGTTGTTTCCTCCTTCCAGGTCTTCCCTCCT--CCCGGCCCAATGACGGA 363120_ENSMUSG00000025932_MOUSE_8201_8311/1-114 CAACTGTTGTTTCTTCC-TGCAGGTCTATCCTCCTCCCCTCCCCTTGTCAGGA- 363120_ENSRNOG00000007590_RAT_8241_8351/1-114 CAACTGCTGTTTCTTCC-TGCAGGTCTCTCCTCCTCCCCTCCCCTTGTCAAGA- * ** * ****** *** * ******* ****** ** ** * * *
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 114 Mean pairwise identity: 75.78 Mean single sequence MFE: -38.95 Consensus MFE: -19.24 Energy contribution: -21.80 Covariance contribution: 2.56 Mean z-score: -2.20 Structure conservation index: 0.49 SVM decision value: 0.38 SVM RNA-class probability: 0.711207 Prediction: RNA ###################################################################### >363120_ENSG00000104313_HUMAN_8178_8286/1-114 AAAGAGAAAAAGGAGGAUACUGCAGCAGUGAAAGGCAUAUUGUCUUUAAGUUGAGGUCUCAACUGUUGUUUCCUCCUGCAGGUCUAUCCUCCUCCUCCCCUUGUCAGGG ...(((....(((((((((((((((.((.(((((((.....)))....((((((....))))))....)))))).))))))...))))))))).)))((((....)))) ( -39.10) >363120_ENSDARG00000014259_ZEBRAFISH_7958_8067/1-114 AAGGGGAAGAGAGGAGUCGGUGGAACAGAGAGGCACAUUGUCUGUAGGUUCAACCCCUCCACCGUUGUUUCCUCCUUCCAGGUCUUCCCUCCUCCCGGCCCAAUGACGGA .((((((((.((((((.((((((((((((.((.....)).))))).((.....))..)))))))....)))))))))))(((.....)))))).(((.........))). ( -41.70) >363120_ENSMUSG00000025932_MOUSE_8201_8311/1-114 AACGAGAAAAGGGAGGAUACUGCAGCCAGGAAAGGCAUAGUGUCUUUAAGUGGAGGGCUCAACUGUUGUUUCUUCCUGCAGGUCUAUCCUCCUCCCCUCCCCUUGUCAGGA ...(((....(((((((((((((((..(((((..(((..(.((((((.....)))))).)...)))..)))))..))))))...)))))))))...))).(((....))). ( -37.90) >363120_ENSRNOG00000007590_RAT_8241_8351/1-114 AAAGAGAAAAGGGAGGAUACUGCGGCCAGGAAAGGCAUAGUAUCUUUAAGUGGAGGGCUCAACUGCUGUUUCUUCCUGCAGGUCUCUCCUCCUCCCCUCCCCUUGUCAAGA ...((.((..((((((((((((..(((......))).))))))).....(.((((((.....((((...........)))).....)))))).)..))))).)).)).... ( -37.10) >consensus AAAGAGAAAAAGGAGGAUACUGCAGCAAGGAAAGGCAUAGUGUCUUUAAGUG_GAGGGCUCAACUGUUGUUUCCUCC_UGCAGGUCUAUCCUCCU__CCUCCCCUUGUCAGGA_ ...(((....((((((((.((((((.((((((..(((....((((((......)))))).....)))..)))))).).)))))....))))))))...)))............. (-19.24 = -21.80 + 2.56)
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