CLUSTAL W(1.81) multiple sequence alignment 300313_ENSG00000108001_HUMAN_9433_9523/1-93 -CGTCACCCTCTGACAGCGCCGCTCCCGGGGGCTTCGAGTTTT-GGCTCCCGGGAAAGGG 300313_ENSRNOG00000016102_RAT_9424_9514/1-93 -CGTCACCCTCTGACAGCGCCGCTCCCGGGGGCTTCGAGTTTT-GGCTCCCGGGAAAGGG 300313_ENSMUSG00000010476_MOUSE_9424_9514/1-93 -CGTCACCCTCTGACAGCGCCGCTCCCGGGGGCTTCGAGTTTT-GGCTCCCGGGAAAGGG 300313_SINFRUG00000145103_FUGU_9333_9423/1-93 GCGTCATATTCTGACAGCGCCTCTCCCGTGGGTTTAGAGTTTT-GGCTCCCGGGAAAGAT 300313_ENSDARG00000003613_ZEBRAFISH_9346_9438/1-93 GCGTCATCCTCTGACAGCGTGGATCCCGTGGGTCTGGAGTTTTTGGCTCCCGGGAAAGGT ***** ********** ***** *** * ******* ************** 300313_ENSG00000108001_HUMAN_9433_9523/1-93 TCCATTCCTCGGGGAGAGAGGGCTGAGTTTTGT 300313_ENSRNOG00000016102_RAT_9424_9514/1-93 TCCATTCCTCGGGGAGAGAGGGCTGAGTTTTGT 300313_ENSMUSG00000010476_MOUSE_9424_9514/1-93 TCCATTCCTCGGGGAGAGAGGGCTGAGTTTTGT 300313_SINFRUG00000145103_FUGU_9333_9423/1-93 TTCAGTCCTCGGGGAAAGAGGGCTTAGTTTTG- 300313_ENSDARG00000003613_ZEBRAFISH_9346_9438/1-93 TCCAGTCCTGGGGGATAGAGGGTTGAGTTTTGT * ** **** ***** ****** * ******* //
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 93 Mean pairwise identity: 88.49 Mean single sequence MFE: -37.70 Consensus MFE: -30.78 Energy contribution: -31.02 Covariance contribution: 0.24 Mean z-score: -1.47 Structure conservation index: 0.82 SVM decision value: 0.19 SVM RNA-class probability: 0.628126 Prediction: RNA ###################################################################### >300313_ENSG00000108001_HUMAN_9433_9523/1-93 CGUCACCCUCUGACAGCGCCGCUCCCGGGGGCUUCGAGUUUUGGCUCCCGGGAAAGGGUCCAUUCCUCGGGGAGAGAGGGCUGAGUUUUGU ..(((((((((....((.((..(((((((((((.........)))))))))))..))))...((((....)))))))))).)))....... ( -41.50) >300313_ENSRNOG00000016102_RAT_9424_9514/1-93 CGUCACCCUCUGACAGCGCCGCUCCCGGGGGCUUCGAGUUUUGGCUCCCGGGAAAGGGUCCAUUCCUCGGGGAGAGAGGGCUGAGUUUUGU ..(((((((((....((.((..(((((((((((.........)))))))))))..))))...((((....)))))))))).)))....... ( -41.50) >300313_ENSMUSG00000010476_MOUSE_9424_9514/1-93 CGUCACCCUCUGACAGCGCCGCUCCCGGGGGCUUCGAGUUUUGGCUCCCGGGAAAGGGUCCAUUCCUCGGGGAGAGAGGGCUGAGUUUUGU ..(((((((((....((.((..(((((((((((.........)))))))))))..))))...((((....)))))))))).)))....... ( -41.50) >300313_SINFRUG00000145103_FUGU_9333_9423/1-93 GCGUCAUAUUCUGACAGCGCCUCUCCCGUGGGUUUAGAGUUUUGGCUCCCGGGAAAGAUUUCAGUCCUCGGGGAAAGAGGGCUUAGUUUUG ((((((.....)))).)).....(((((.(((((.........))))).)))))((((((..(((((((.......))))))).)))))). ( -29.70) >300313_ENSDARG00000003613_ZEBRAFISH_9346_9438/1-93 GCGUCAUCCUCUGACAGCGUGGAUCCCGUGGGUCUGGAGUUUUUGGCUCCCGGGAAAGGUUCCAGUCCUGGGGGAUAGAGGGUUGAGUUUUGU ...((((((((((..(((.(((((((...)))))))..))).....(((((((((..(....)..))))))))).))))))).)))....... ( -34.30) >consensus _CGUCACCCUCUGACAGCGCCGCUCCCGGGGGCUUCGAGUUUU_GGCUCCCGGGAAAGGGUCCAUUCCUCGGGGAGAGAGGGCUGAGUUUUGU ...(((((((((.......((..(((((((((((..........)))))))))))..)).....((((....)))))))))).)))....... (-30.78 = -31.02 + 0.24)
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