CLUSTAL W(1.81) multiple sequence alignment 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 GTCTTCTTTGACGGTTT--CCAGGATAAGTTTGGCCACTGAGTCACTC-TT--GATGTCT 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 TTCAGATTTTA--GTCTGATTAAAGTATTTAGCTGGGCTTTATCTTTA-CG--CTTGTCT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 CTCTTCTTTAACGGTCT--CAACTATAAGTTTCACAGAGTTGTTGGCT-TT--GTATTCC 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 CCCTGCTTTCC--TCCT--TTACTGTTTCTACAATTAACTTGACAGCA-TT--ATTCGCT 280356_ENSG00000183788_HUMAN_879_1577/1-737 TCCCGTTGCTC--ATCT--TCACTGGGTCGCCCTTTTCTTTCTCTGCACCGTCCTTGAAT * * * * 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 CTGTAGTCTGTCACT-GA---GTCC-CAGC-----TATCCTCTCCTTTGAAATCAAAAGA 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 CGCTCTT-TGTATCTGTCTATATCCTTTTTATCTTTCTCTTTTCCAT---CTG---GAGT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 TTGTACTCTTTGACAGGA-GTGTCC-CAGC-----TGTCGTCTCCATAAAGGG---AGGA 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 TTGTATTCTTTAACCGGG-GTATCC-CAGC-----TATCATCTCCATACAATG---AACT 280356_ENSG00000183788_HUMAN_879_1577/1-737 TTCTCCT-TCAGTTTGGC-ATCGCC-GAGCCTCGGGCCCTCGTCCCTGGACTT---GTCT * * * ** * *** * 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 GGAGTCTGACAGGTCAGAAAACCACCTCTCTTGCTGGTCATCAGAGAGGCTGAACTTGGT 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 TTTCAAAGGCTCATC-TCTTGCCCTT-TCTTTAAGGGACAGAGCTTTCTCATGCTGGGTT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 GTCGGATGACATCTCTGAACCCCATCTGTCATGATGGTCATCTGAGGCACTGAGTTTTGT 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 GTCAGAAGACATATCCGACTGCCACCGATCATGGGGGTCATCTGATGCACTCATCTTGGT 280356_ENSG00000183788_HUMAN_879_1577/1-737 TTGAGCACGCGGGGCGGGCTGTCCTTGTCCCTGGTGGCGGGCTTCTNNNNNNNNNNNNNN * * * * ** 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 GTCCTCGTGGTCCAGAAACTGGCTTTTGTTACAG--TAGTCGTCAAAAGC--AGCGTCGT 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 TT-ACCACTG-CTGTCTC-CTGTACTGGACTTTCTCTCTTCCTGCAAATG--TTTCAAGT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 GTCCTCCATGTTGAGAAAGCGGTTGTTGTCATAG--CTTTCATACTCTGA--CTCCTCTT 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 GT-CTTCAAG-TAAGTGA-TTTTTTAAGTCAAAG--TCGTCATAGTCAGG--TTCCTCCT 280356_ENSG00000183788_HUMAN_879_1577/1-737 NN-NNNNNNN-NNNNNNN-NNNNNNNNNNNNNNNNCAGCCCATCCGCATGCCTGTCCCGG * * 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 CCCTGCACAGCTTTTCCTTCTTGAGCTTCTCTTTGTCCTCTCTGAAGGCCTTCTC--CTT 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 -TAGTTACATTTACACTTTCTTTATCTCTAAGTTTCTCCTTCTTCTCTCCTTCCCTGTCT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 -TCTGTGC-TTTAT-CTTTTTTACCTTTCTCTTCTTTTGCATTTTTCTCCTTGTCCACCT 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 -T-GATGCTTTTATCCCTCTTAGACTTTTCCTTCTCTTCTTTGACACT-TTTCTCAGATT 280356_ENSG00000183788_HUMAN_879_1577/1-737 -TGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 300 Mean pairwise identity: 37.40 Mean single sequence MFE: -62.86 Consensus MFE: -4.74 Energy contribution: -4.46 Covariance contribution: -0.28 Mean z-score: -1.87 Structure conservation index: 0.08 SVM decision value: 0.17 SVM RNA-class probability: 0.616746 Prediction: RNA ###################################################################### >280356_ENSRNOG00000027886_RAT_3595_4278/1-737 GUCUUCUUUGACGGUUUCCAGGAUAAGUUUGGCCACUGAGUCACUCUUGAUGUCUCUGUAGUCUGUCACUGAGUCCCAGCUAUCCUCUCCUUUGAAAUCAAAAGAGGAGUCUGACAGGUCAGAAAACCACCUCUCUUGCUGGUCAUCAGAGAGGCUGAACUUGGUGUCCUCGUGGUCCAGAAACUGGCUUUUGUUACAGUAGUCGUCAAAAGCAGCGUCGUCCCUGCACAGCUUUUCCUUCUUGAGCUUCUCUUUGUCCUCUCUGAAGGCCUUCUCCUU ((((((((((((((((((..(((((((((..(((((.(((.(((....).)).))).))......((.((((...(((((..((((((..((((....)))))))))).(((((....)))))..............)))))...)))).)))))..))))))....((....))))).))))))((((.(((...))).)))))))))).((((.(....).))))..(((((.........)))))................))))))......... ( -82.10) >280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 UUCAGAUUUUAGUCUGAUUAAAGUAUUUAGCUGGGCUUUAUCUUUACGCUUGUCUCGCUCUUUGUAUCUGUCUAUAUCCUUUUUAUCUUUCUCUUUUCCAUCUGGAGUUUUCAAAGGCUCAUCUCUUGCCCUUUCUUUAAGGGACAGAGCUUUCUCAUGCUGGGUUUUACCACUGCUGUCUCCUGUACUGGACUUUCUCUCUUCCUGCAAAUGUUUCAAGUUAGUUACAUUUACACUUUCUUUAUCUCUAAGUUUCUCCUUCUUCUCUCCUUCCCUGUCU .((((((....))))))..((((((((((((((((((((........((.......)).....................................((((....))))......)))))))).((((..(((((.....)))))..)))).........)))))))....(((.(((........))).)))))))).........((.((((((..(......)..)))))).))............................................. ( -44.40) >280356_SINFRUG00000125527_FUGU_4384_5074/1-737 CUCUUCUUUAACGGUCUCAACUAUAAGUUUCACAGAGUUGUUGGCUUUGUAUUCCUUGUACUCUUUGACAGGAGUGUCCCAGCUGUCGUCUCCAUAAAGGGAGGAGUCGGAUGACAUCUCUGAACCCCAUCUGUCAUGAUGGUCAUCUGAGGCACUGAGUUUUGUGUCCUCCAUGUUGAGAAAGCGGUUGUUGUCAUAGCUUUCAUACUCUGACUCCUCUUUCUGUGCUUUAUCUUUUUUACCUUUCUCUUCUUUUGCAUUUUUCUCCUUGUCCACCU ............(((......((((((....(((((((.....)))))))....))))))......((((((((.......((.(....)..((((.((((((((((((((.(((((...(((...(((((......))))))))...(((((((........))).)))).)))))..((((((...((....))..))))))....)))))))))))))).)))).............................))......)))).)))).))). ( -71.32) >280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 CCCUGCUUUCCUCCUUUACUGUUUCUACAAUUAACUUGACAGCAUUAUUCGCUUUGUAUUCUUUAACCGGGGUAUCCCAGCUAUCAUCUCCAUACAAUGAACUGUCAGAAGACAUAUCCGACUGCCACCGAUCAUGGGGGUCAUCUGAUGCACUCAUCUUGGUGUCUUCAAGUAAGUGAUUUUUUAAGUCAAAGUCGUCAUAGUCAGGUUCCUCCUUGAUGCUUUUAUCCCUCUUAGACUUUUCCUUCUCUUCUUUGACACUUUUCUCAGAUU ..(((......((.((((((................((((((........(((..(((((((......)))))))...)))..((((.........)))).))))))((((((((....((((.(((.......))).))))....((((....))))...)))))))).)))))).))........(((((((..(....(((((((.....))).((((....)))).......)))).........)..)))))))........)))... ( -54.92) >280356_ENSG00000183788_HUMAN_879_1577/1-737 UCCCGUUGCUCAUCUUCACUGGGUCGCCCUUUUCUUUCUCUGCACCGUCCUUGAAUUUCUCCUUCAGUUUGGCAUCGCCGAGCCUCGGGCCCUCGUCCCUGGACUUGUCUUUGAGCACGCGGGGCGGGCUGUCCUUGUCCCUGGUGGCGGGCUUCUNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCCCAUCCGCAUGCCUGUCCCGGUGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN ...(((.(((((....((..(((..((((......................((((.......))))(((((((...)))))))...)))).)))(((....))).))....))))))))(((((((((((((((........)).((.(((((..................................................)))))..))))).))))))))))............................................................ ( -61.56) >consensus CUCUGCUUUUA__GUCU__UCACUGUAUCUACCACUACUUUGUCAGCA_UU__CUUGUCUUUGUACUCUGUAACUGGA_AUAUCC_CAGC_____UAUCUUCUCCAUAGAAUG___AAGUGUCAGAAGACAGAUC_GAAUGCCAUCGUUCAUGAUGGUCAUCUGAUGCACUGA_CUUGGUGU_CUCCAUG_C_AGAAA_CGGUUUUUGUCAUAG__UCGUCAUACACAGG__UUCCUCGU_UCGGUAC_UUUAU_CCUUCUUGACCUUCUCUUCUCUCCCUCUUCACUCCUUCUC__CCU ....................................................................................................................................(((.((..((((((......))))))..)).)))...................................................................................................................................... ( -4.74 = -4.46 + -0.28)
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