CLUSTAL W(1.81) multiple sequence alignment 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 TCTCCTTTGAAATCAAAAGAGGAGTCTGACAGGTCAGAAAACCACCTCTCTTGCTGGTCA 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 TTTCCAT---CTG---GAGTTTTCAAAGGCTCATC-TCTTGCCCTT-TCTTTAAGGGACA 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 TCTCCATAAAGGG---AGGAGTCGGATGACATCTCTGAACCCCATCTGTCATGATGGTCA 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 TCTCCATACAATG---AACTGTCAGAAGACATATCCGACTGCCACCGATCATGGGGGTCA 280356_ENSG00000183788_HUMAN_879_1577/1-737 CGTCCCTGGACTT---GTCTTTGAGCACGCGGGGCGGGCTGTCCTTGTCCCTGGTGGCGG *** * * * * * ** 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 TCAGAGAGGCTGAACTTGGTGTCCTCGTGGTCCAGAAACTGGCTTTTGTTACAG--TAGT 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 GAGCTTTCTCATGCTGGGTTTT-ACCACTG-CTGTCTC-CTGTACTGGACTTTCTCTCTT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 TCTGAGGCACTGAGTTTTGTGTCCTCCATGTTGAGAAAGCGGTTGTTGTCATAG--CTTT 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 TCTGATGCACTCATCTTGGTGT-CTTCAAG-TAAGTGA-TTTTTTAAGTCAAAG--TCGT 280356_ENSG00000183788_HUMAN_879_1577/1-737 GCTTCTNNNNNNNNNNNNNNNN-NNNNNNN-NNNNNNN-NNNNNNNNNNNNNNNNCAGCC 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 CGTCAAAAGC--AGCGTCGTCCCTGCACAGCTTTTCCTTCTTGAGCTTCTCTTTGTCCTC 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 CCTGCAAATG--TTTCAAGT-TAGTTACATTTACACTTTCTTTATCTCTAAGTTTCTCCT 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 CATACTCTGA--CTCCTCTT-TCTGTGC-TTTAT-CTTTTTTACCTTTCTCTTCTTTTGC 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 CATAGTCAGG--TTCCTCCT-T-GATGCTTTTATCCCTCTTAGACTTTTCCTTCTCTTCT 280356_ENSG00000183788_HUMAN_879_1577/1-737 CATCCGCATGCCTGTCCCGG-TGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN * * 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 TCTGAAGGCCTTCTC--CTTCTCCTTGGAGA-CCTCCTTCATGTCG-TTCTTCTTTT--C 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 TCTTCTCTCCTTCCCTGTCTCTGTCTTTAGTTCTGACCTTCTCTGATTTGGAATACT--C 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 ATTTTTCTCCTTGTCCACCTTTAAATGCTTTTCCTCTTTCTGCTCA-CTTTTCCTGTCAG 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 TTGACACT-TTTCTCAGATTTAAAATGTTTTTCTTCTTTTTGCTCA-TTCTTTTTGT--C 280356_ENSG00000183788_HUMAN_879_1577/1-737 NNNNNNNNNNNNNNNNNNNNTAGGATGTTGATGGCACTAGATCCATAAGGCTTTAGTTCC * * 280356_ENSRNOG00000027886_RAT_3595_4278/1-737 -TAGCTTGAACGGTC--GATCTTTGGACTTTTTCTTCCTTTCTTCTTTGTACAGTCTTAA 280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 ACTGCTGTCA-GATTGCTTTCTTTTCTCTAAGTGCTTTTCTTTGTTTTCTGCTAAGTACC 280356_SINFRUG00000125527_FUGU_4384_5074/1-737 CCTTAGAGGATGATT--TTTCTTTAGATTTCTTCTTTTTATCCTCTTTGTGAGCCTTTTG 280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 AGTTTTAAGA-GATT--TATCTTTTGACTTCTTCTTTTTATCATCTTTATGGGCTTTTTG 280356_ENSG00000183788_HUMAN_879_1577/1-737 TTTTCTATTT-TCTTGGAAGGTTCTCTCTCGGAATCATTTTTATCTTTCTTTTCGGTAGA * ** * * * *** * *
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 300 Mean pairwise identity: 38.14 Mean single sequence MFE: -63.28 Consensus MFE: -4.74 Energy contribution: -4.46 Covariance contribution: -0.28 Mean z-score: -2.67 Structure conservation index: 0.07 SVM decision value: 0.27 SVM RNA-class probability: 0.664130 Prediction: RNA ###################################################################### >280356_ENSRNOG00000027886_RAT_3595_4278/1-737 UCUCCUUUGAAAUCAAAAGAGGAGUCUGACAGGUCAGAAAACCACCUCUCUUGCUGGUCAUCAGAGAGGCUGAACUUGGUGUCCUCGUGGUCCAGAAACUGGCUUUUGUUACAGUAGUCGUCAAAAGCAGCGUCGUCCCUGCACAGCUUUUCCUUCUUGAGCUUCUCUUUGUCCUCUCUGAAGGCCUUCUCCUUCUCCUUGGAGACCUCCUUCAUGUCGUUCUUCUUUUCUAGCUUGAACGGUCGAUCUUUGGACUUUUUCUUCCUUUCUUCUUUGUACAGUCUUAA .(((.((((....)))).)))(((.((((((((..(((((((((.(......).))))....(((((((((.((..(((...((....)).)))((((((((((((((..((....))...))))))).(((.......))).)))..))))....)).)))))))))..((((....((((((...(((((........)))))...))))))((((((((..((.....))...)))))).))......))))..........)))))..))))).))).))).. ( -77.10) >280356_ENSMUSG00000034647_MOUSE_1056_1743/1-737 UUUCCAUCUGGAGUUUUCAAAGGCUCAUCUCUUGCCCUUUCUUUAAGGGACAGAGCUUUCUCAUGCUGGGUUUUACCACUGCUGUCUCCUGUACUGGACUUUCUCUCUUCCUGCAAAUGUUUCAAGUUAGUUACAUUUACACUUUCUUUAUCUCUAAGUUUCUCCUUCUUCUCUCCUUCCCUGUCUCUGUCUUUAGUUCUGACCUUCUCUGAUUUGGAAUACUCACUGCUGUCAGAUUGCUUUCUUUUCUCUAAGUGCUUUUCUUUGUUUUCUGCUAAGUACC ........(((((......(((((..((((.....((((.....))))((((((((........)))((((....(((.(((........))).))).....................((((((((((((..........((((...........)))).......................(((.(((....)))....))).....))))))))))))))))....))))))))).))))).....))))).((((((..(..........)..)))))). ( -47.50) >280356_SINFRUG00000125527_FUGU_4384_5074/1-737 UCUCCAUAAAGGGAGGAGUCGGAUGACAUCUCUGAACCCCAUCUGUCAUGAUGGUCAUCUGAGGCACUGAGUUUUGUGUCCUCCAUGUUGAGAAAGCGGUUGUUGUCAUAGCUUUCAUACUCUGACUCCUCUUUCUGUGCUUUAUCUUUUUUACCUUUCUCUUCUUUUGCAUUUUUCUCCUUGUCCACCUUUAAAUGCUUUUCCUCUUUCUGCUCACUUUUCCUGUCAGCCUUAGAGGAUGAUUUUUCUUUAGAUUUCUUCUUUUUAUCCUCUUUGUGAGCCUUUUG ....((((.((((((((((((((.(((((...(((...(((((......))))))))...(((((((........))).)))).)))))..((((((...((....))..))))))....)))))))))))))).)))).............................((((((..................)))))).............((((((................(((((((((.........(((.....)))..)))))))))..))))))...... ( -72.24) >280356_ENSDARG00000015393_ZEBRAFISH_5389_6069/1-737 UCUCCAUACAAUGAACUGUCAGAAGACAUAUCCGACUGCCACCGAUCAUGGGGGUCAUCUGAUGCACUCAUCUUGGUGUCUUCAAGUAAGUGAUUUUUUAAGUCAAAGUCGUCAUAGUCAGGUUCCUCCUUGAUGCUUUUAUCCCUCUUAGACUUUUCCUUCUCUUCUUUGACACUUUUCUCAGAUUUAAAAUGUUUUUCUUCUUUUUGCUCAUUCUUUUUGUCAGUUUUAAGAGAUUUAUCUUUUGACUUCUUCUUUUUAUCAUCUUUAUGGGCUUUUUG ..((((((..((((((((.((((((((((....((((.(((.......))).))))....((((....))))...))))))))(((.(((.(((.((((((((((((((.((((..((((((......))))))...............(((........)))......))))))))).....))))))))).)))...))))))...............)).)))).((((((((....)))))))).............))))...))))))....... ( -57.90) >280356_ENSG00000183788_HUMAN_879_1577/1-737 CGUCCCUGGACUUGUCUUUGAGCACGCGGGGCGGGCUGUCCUUGUCCCUGGUGGCGGGCUUCUNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCAGCCCAUCCGCAUGCCUGUCCCGGUGCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNUAGGAUGUUGAUGGCACUAGAUCCAUAAGGCUUUAGUUCCUUUUCUAUUUUCUUGGAAGGUUCUCUCUCGGAAUCAUUUUUAUCUUUCUUUUCGGUAGA .(((....)))...(((((((((((.(((((((((((((((........)).((.(((((..................................................)))))..))))).)))))))))))))).........................................................((((((..(((((.((((((.((....)).)))))).))..(((((......)))))((((((.....)))))))))..)))))).....)))).))) ( -61.66) >consensus UCUCCAUAGAAUG___AAGUGUCAGAAGACAGAUC_GAAUGCCAUCGUUCAUGAUGGUCAUCUGAUGCACUGA_CUUGGUGU_CUCCAUG_C_AGAAA_CGGUUUUUGUCAUAG__UCGUCAUACACAGG__UUCCUCGU_UCGGUAC_UUUAU_CCUUCUUGACCUUCUCUUCUCUCCCUCUUCACUCCUUCUC__CCUUUAAAUGUUGUUCCUCCUUCAUCUCA_UUCUUCUUGU__CACUUCUAGGA_GAUU__UAUCUUUUGACUUCUUCUUUUUAUCAUCUUUCUGCGCUGUUUA ................................(((.((..((((((......))))))..)).))).......................................................................................................................................................................................................................................... ( -4.74 = -4.46 + -0.28)
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