CLUSTAL W(1.81) multiple sequence alignment 244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 TGAGAAGGGCTGCGGAAGATAATTTATAGGTTTTAATTACTCTTCATTCCGCCTGATCAG 244427_ENSRNOG00000002457_RAT_6069_6575/1-510 TGAGAAGGGCTGCGGAAGATAATTTATAGGTTTTAATTACTCTTCATTCCGCCTGATCAG 244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 TGAGAGGGACTGCAAAAGATAATTTATAGGTTTTAATTAGCGCCCATTCCGCCTGATCAG 244427_ENSG00000163064_HUMAN_6937_7444/1-510 CGAGAAGGGCTGCGGAAGATAATTTATAGGTTTTAATTACTCTTCATTCCGCCTGATCAG **** ** **** ************************ **************** 244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 CTTGGCGTTCATCACAGGCCTGTGAATAAAACCCTGGTCAAAAGCTCTGTCACATAGCAC 244427_ENSRNOG00000002457_RAT_6069_6575/1-510 CTTGGCGTTCATCACAGGCCTGTGAATAAAACCCTGGTCAAAAGCTCTGTCACATAGCGC 244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 CTTGGCGTTCATCACTAGCGAGTGAATAAAAGCC-GGTCAAAAGCACTGTCACTACCCGC 244427_ENSG00000163064_HUMAN_6937_7444/1-510 CTTGGCGTTCATCACAGGCCTGTGAATAAAACCCTGGTCAAAAGCTCTGTCACATCGCGC *************** ** ********** ** ********** ******* * * 244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 TGGCAAGACGTTTAATCAAAGTGA-NNNNNNNNNNNNNNNNNNNNNNNNNNTCCTGTACG 244427_ENSRNOG00000002457_RAT_6069_6575/1-510 TGGCAAGACGTTTAATCAAAGTGA-NNNNNNNNNNNNNNNNNNNNNNNNNNTCCTGTACG 244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 TGGCAGGGCGCTTTATCAAGGTAAGCAGGGCCGTATTACACGAGGTGTAGA--GGCAACG 244427_ENSG00000163064_HUMAN_6937_7444/1-510 TGGCAAGACGCTTAATCAAAGTGANNNNNNNNNNNNNNNNNNNNNNNNNNNTCCTCCACG ***** * ** ** ***** ** * *** 244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 TAATTTCCAGCCAGCTGATAAAGCCAGCTGAATAATGCGGCGGCCCTTTGGAGACACCAT 244427_ENSRNOG00000002457_RAT_6069_6575/1-510 TAATTTCCAGCCAGCTGATAAAGCCAGCTGAATAATGCGGCGGCCCTTTGGAGACACCAT 244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 TAATTTCCCCAGTGCTGATAAAGCCCGTTGAATAATGCCGGAGTCATCT-GAGACACCAT 244427_ENSG00000163064_HUMAN_6937_7444/1-510 TAATTTCCAGCCAGCTGATAAAGCCAGCTGAATAATACGGCGGCCCTTTGGAGACACCAT ******** ************ * ******** * * * * * * ********** 244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 TTACAGAAATGACTTTATTGACGGCTTAACGTCGGTAATTCATTTTACCTTTCATGTAGT 244427_ENSRNOG00000002457_RAT_6069_6575/1-510 TTACAGAAATGACTTTATTGACGGCTTAACGTCGGTAATTCATTTTACCTTTCATGTAGT 244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 TTACTGAAATGACTTTATTGACGACTTAACGTCGGTAATTCATTTT-CCCTTCACGTATC 244427_ENSG00000163064_HUMAN_6937_7444/1-510 TTACAGAAATGACTTTATTGACGGCTTAACGTCGGTAATTCATTTTACCTTTCATGTAGT **** ****************** ********************** ** **** ***
############################ RNAz 0.1 ############################## Sequences: 4 Columns: 300 Mean pairwise identity: 85.77 Mean single sequence MFE: -80.82 Consensus MFE: -61.47 Energy contribution: -61.97 Covariance contribution: 0.50 Mean z-score: -1.38 Structure conservation index: 0.76 SVM decision value: -0.06 SVM RNA-class probability: 0.505275 Prediction: RNA ###################################################################### >244427_ENSMUSG00000036888_MOUSE_6983_7489/1-510 UGAGAAGGGCUGCGGAAGAUAAUUUAUAGGUUUUAAUUACUCUUCAUUCCGCCUGAUCAGCUUGGCGUUCAUCACAGGCCUGUGAAUAAAACCCUGGUCAAAAGCUCUGUCACAUAGCACUGGCAAGACGUUUAAUCAAAGUGANNNNNNNNNNNNNNNNNNNNNNNNNNUCCUGUACGUAAUUUCCAGCCAGCUGAUAAAGCCAGCUGAAUAAUGCGGCGGCCCUUUGGAGACACCAUUUACAGAAAUGACUUUAUUGACGGCUUAACGUCGGUAAUUCAUUUUACCUUUCAUGUAGU ...((((((((((.((((((((((..........))))).)))))....(((....((((((.(((..((((((((....)))))........((((......(((.((...)).))).((((....((((.............................................)))).....)))))))).)))....))))))))).....)))))))))))))(....).....((((((((((((..(((((((((......))))))))).)))))))........))))). ( -77.78) >244427_ENSRNOG00000002457_RAT_6069_6575/1-510 UGAGAAGGGCUGCGGAAGAUAAUUUAUAGGUUUUAAUUACUCUUCAUUCCGCCUGAUCAGCUUGGCGUUCAUCACAGGCCUGUGAAUAAAACCCUGGUCAAAAGCUCUGUCACAUAGCGCUGGCAAGACGUUUAAUCAAAGUGANNNNNNNNNNNNNNNNNNNNNNNNNNUCCUGUACGUAAUUUCCAGCCAGCUGAUAAAGCCAGCUGAAUAAUGCGGCGGCCCUUUGGAGACACCAUUUACAGAAAUGACUUUAUUGACGGCUUAACGUCGGUAAUUCAUUUUACCUUUCAUGUAGU ...((((((((((.((((((((((..........))))).)))))....(((....((((((.(((.....(((((....)))))...........((((...(((.((...)).)))((((((..((.(((.......((.((..........................))))......))).))..))))))))))...))))))))).....)))))))))))))(....).....((((((((((((..(((((((((......))))))))).)))))))........))))). ( -80.59) >244427_ENSDARG00000015073_ZEBRAFISH_4427_4928/1-510 UGAGAGGGACUGCAAAAGAUAAUUUAUAGGUUUUAAUUAGCGCCCAUUCCGCCUGAUCAGCUUGGCGUUCAUCACUAGCGAGUGAAUAAAAGCCGGUCAAAAGCACUGUCACUACCCGCUGGCAGGGCGCUUUAUCAAGGUAAGCAGGGCCGUAUUACACGAGGUGUAGAGGCAACGUAAUUUCCCCAGUGCUGAUAAAGCCCGUUGAAUAAUGCCGGAGUCAUCUGAGACACCAUUUACUGAAAUGACUUUAUUGACGACUUAACGUCGGUAAUUCAUUUUCCCUUCACGUAUC (((.(((((...((..((((........(((.(((.(((((((((.....((.(((((.((((...(((((((......)).)))))..)))).)))))...))..(((((........)))))))))((((.((....)))))).((((..((((((((..((......(....).......))...))).)))))..))))))))).))).)))((.(((......))).))))))..))((((((..(((((((((......))))))))).))))))))))))))...... ( -85.12) >244427_ENSG00000163064_HUMAN_6937_7444/1-510 CGAGAAGGGCUGCGGAAGAUAAUUUAUAGGUUUUAAUUACUCUUCAUUCCGCCUGAUCAGCUUGGCGUUCAUCACAGGCCUGUGAAUAAAACCCUGGUCAAAAGCUCUGUCACAUCGCGCUGGCAAGACGCUUAAUCAAAGUGANNNNNNNNNNNNNNNNNNNNNNNNNNNUCCUCCACGUAAUUUCCAGCCAGCUGAUAAAGCCAGCUGAAUAAUACGGCGGCCCUUUGGAGACACCAUUUACAGAAAUGACUUUAUUGACGGCUUAACGUCGGUAAUUCAUUUUACCUUUCAUGUAGU ...(((((((((((((.((.........(((((((..............((((..........))))....(((((....))))).))))))).((((...(((((((((((........)))).))).)))).)))).................................)).))).((((.....((((..(((.....)))..)))).....))))))))))))))(....).....((((((((((((..(((((((((......))))))))).)))))))........))))). ( -79.80) >consensus UGAGAAGGGCUGCGGAAGAUAAUUUAUAGGUUUUAAUUACUCUUCAUUCCGCCUGAUCAGCUUGGCGUUCAUCACAGGCCUGUGAAUAAAACCCUGGUCAAAAGCUCUGUCACAUAGCGCUGGCAAGACGCUUAAUCAAAGUGA___________________________UCCUCUACGUAAUUUCCAGCCAGCUGAUAAAGCCAGCUGAAUAAUGCGGCGGCCCUUUGGAGACACCAUUUACAGAAAUGACUUUAUUGACGGCUUAACGUCGGUAAUUCAUUUUACCUUUCAUGUAGU .((((((((((((....(((........(((((((..............((((..........))))....(((((....))))).)))))))........(((((((((((........)))).))).)))).))).........................................((((.....((((..(((.....)))..)))).....))))))))))))))................(((((((..(((((((((......))))))))).))))))).....))....... (-61.47 = -61.97 + 0.50)
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