CLUSTAL W(1.81) multiple sequence alignment 233826_ENSG00000154358_HUMAN_506_591/1-86 TTCCCGTCCTTGTACCACGTCACCTCTGTCTGGGCCTGGGCCACCTCGCAGCTCAGCGTG 233826_ENSMUSG00000020452_MOUSE_3372_3457/1-86 TTCCCGTCCTTGAACCATGTCACCTCAGTCTGGGCCTGGGCCACCTCGCAGCTCAGTGTG 233826_ENSRNOG00000008975_RAT_3479_3564/1-86 TTCCCATCCTTGAACCATGTCACCTCAGTCTGGGCCTGGGCCACCTCACAGCTCAGTGTG 233826_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 -TCTTATTTTTCCTCCAGATGACTTTGGCTCCAGACTTGGCAAGTTCACACCGAAACACT 233826_SINFRUG00000147616_FUGU_1538_1623/1-86 TTGCCATCTTTGTACCATTTCACTTCTGTTTTCCTATCGGCTACTTCACAGCTCAGAGTG * * ** *** * ** * * * *** * ** ** * * 233826_ENSG00000154358_HUMAN_506_591/1-86 GCAATGGCCCCCGCCTCGGCCTGCAC 233826_ENSMUSG00000020452_MOUSE_3372_3457/1-86 GCACTGGCCCCCGCCTCTGCCTTCAC 233826_ENSRNOG00000008975_RAT_3479_3564/1-86 GCACTGGCCCCGGCCTCTGCCTTCAC 233826_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 GCATTTTCCCCAGTTTCAGTTTCCA- 233826_SINFRUG00000147616_FUGU_1538_1623/1-86 GCTCTCTGGGACAAGATGGCTTTCAC ** * * * **
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 86 Mean pairwise identity: 65.70 Mean single sequence MFE: -22.72 Consensus MFE: -13.72 Energy contribution: -13.24 Covariance contribution: -0.48 Mean z-score: -1.27 Structure conservation index: 0.60 SVM decision value: 0.08 SVM RNA-class probability: 0.571552 Prediction: RNA ###################################################################### >233826_ENSG00000154358_HUMAN_506_591/1-86 UUCCCGUCCUUGUACCACGUCACCUCUGUCUGGGCCUGGGCCACCUCGCAGCUCAGCGUGGCAAUGGCCCCCGCCUCGGCCUGCAC ...........................(((.((((..((((((((.(((......))).))...))))))..)))).)))...... ( -28.20) >233826_ENSMUSG00000020452_MOUSE_3372_3457/1-86 UUCCCGUCCUUGAACCAUGUCACCUCAGUCUGGGCCUGGGCCACCUCGCAGCUCAGUGUGGCACUGGCCCCCGCCUCUGCCUUCAC ...........................((..((((..((((((((.(((......))).))...))))))..))))..))...... ( -23.90) >233826_ENSRNOG00000008975_RAT_3479_3564/1-86 UUCCCAUCCUUGAACCAUGUCACCUCAGUCUGGGCCUGGGCCACCUCACAGCUCAGUGUGGCACUGGCCCCGGCCUCUGCCUUCAC ...........................((..(((((.((((((((.(((......))).))...)))))).)))))..))...... ( -28.70) >233826_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 UCUUAUUUUUCCUCCAGAUGACUUUGGCUCCAGACUUGGCAAGUUCACACCGAAACACUGCAUUUUCCCCAGUUUCAGUUUCCA ................(..((((..((((...((....(((..(((.....)))....)))....))...))))..))))..). ( -8.90) >233826_SINFRUG00000147616_FUGU_1538_1623/1-86 UUGCCAUCUUUGUACCAUUUCACUUCUGUUUUCCUAUCGGCUACUUCACAGCUCAGAGUGGCUCUCUGGGACAAGAUGGCUUUCAC ..((((((((.((.((......................((((.......))))(((((.....)))))))))))))))))...... ( -23.90) >consensus UUCCCAUCCUUGAACCAUGUCACCUCAGUCUGGGCCUGGGCCACCUCACAGCUCAGCGUGGCACUGGCCCCCGCCUCGGCCUUCAC ...............................(((((...(((((((........)).)))))...)))))................ (-13.72 = -13.24 + -0.48)
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