CLUSTAL W(1.81) multiple sequence alignment 233460_ENSG00000154358_HUMAN_7192_7277/1-86 TTCCCGTCCTTGTACCACATCACCTCCGTCTGGGCCTGAGCCACCTCGCAGCTGAGTGTG 233460_ENSMUSG00000020452_MOUSE_6160_6245/1-86 TTCCCGTCCTTGAACCATGTCACCTCAGTCTGGGCCTGGGCCACCTCGCAGCTCAGTGTG 233460_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 -TCTTATTTTTCCTCCAGATGACTTTGGCTCCAGACTTGGCAAGTTCACACCGAAACACT 233460_ENSRNOG00000008975_RAT_3479_3564/1-86 TTCCCATCCTTGAACCATGTCACCTCAGTCTGGGCCTGGGCCACCTCACAGCTCAGTGTG 233460_SINFRUG00000147616_FUGU_1538_1623/1-86 TTGCCATCTTTGTACCATTTCACTTCTGTTTTCCTATCGGCTACTTCACAGCTCAGAGTG * * ** *** * ** * * * ** * ** ** * * 233460_ENSG00000154358_HUMAN_7192_7277/1-86 GTGCTGGCCCCCGCCTGGGCCTGCAC 233460_ENSMUSG00000020452_MOUSE_6160_6245/1-86 GCGCTGGCCCCAGCCTCTGCCTTCAC 233460_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 GCATTTTCCCCAGTTTCAGTTTCCA- 233460_ENSRNOG00000008975_RAT_3479_3564/1-86 GCACTGGCCCCGGCCTCTGCCTTCAC 233460_SINFRUG00000147616_FUGU_1538_1623/1-86 GCTCTCTGGGACAAGATGGCTTTCAC * * * * **
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 86 Mean pairwise identity: 63.72 Mean single sequence MFE: -23.70 Consensus MFE: -13.62 Energy contribution: -12.74 Covariance contribution: -0.88 Mean z-score: -1.36 Structure conservation index: 0.57 SVM decision value: 0.24 SVM RNA-class probability: 0.650398 Prediction: RNA ###################################################################### >233460_ENSG00000154358_HUMAN_7192_7277/1-86 UUCCCGUCCUUGUACCACAUCACCUCCGUCUGGGCCUGAGCCACCUCGCAGCUGAGUGUGGUGCUGGCCCCCGCCUGGGCCUGCAC ...........(((((((((((.((.((....(((....)))....)).)).))).)))))))).(((((......)))))..... ( -32.20) >233460_ENSMUSG00000020452_MOUSE_6160_6245/1-86 UUCCCGUCCUUGAACCAUGUCACCUCAGUCUGGGCCUGGGCCACCUCGCAGCUCAGUGUGGCGCUGGCCCCAGCCUCUGCCUUCAC .........................(((.(((((((.(.(((((((........)).))))).).)).)))))...)))....... ( -24.80) >233460_ENSDARG00000022108_ZEBRAFISH_735_818/1-86 UCUUAUUUUUCCUCCAGAUGACUUUGGCUCCAGACUUGGCAAGUUCACACCGAAACACUGCAUUUUCCCCAGUUUCAGUUUCCA ................(..((((..((((...((....(((..(((.....)))....)))....))...))))..))))..). ( -8.90) >233460_ENSRNOG00000008975_RAT_3479_3564/1-86 UUCCCAUCCUUGAACCAUGUCACCUCAGUCUGGGCCUGGGCCACCUCACAGCUCAGUGUGGCACUGGCCCCGGCCUCUGCCUUCAC ...........................((..(((((.((((((((.(((......))).))...)))))).)))))..))...... ( -28.70) >233460_SINFRUG00000147616_FUGU_1538_1623/1-86 UUGCCAUCUUUGUACCAUUUCACUUCUGUUUUCCUAUCGGCUACUUCACAGCUCAGAGUGGCUCUCUGGGACAAGAUGGCUUUCAC ..((((((((.((.((......................((((.......))))(((((.....)))))))))))))))))...... ( -23.90) >consensus UUCCCAUCCUUGAACCAUAUCACCUCAGUCUGGGCCUGGGCCACCUCACAGCUCAGUGUGGCACUGGCCCCAGCCUCGGCCUUCAC ...........................((..(((((...(((((((........)).)))))...)))))..))............ (-13.62 = -12.74 + -0.88)
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