Results for CNB 231552-rev

Input Alignment

CLUSTAL W(1.81) multiple sequence alignment


231552_ENSG00000134107_HUMAN_7051_7118/1-133        GGGAGCGAGTGGGTGGGTTGGAGCTACGTGT----TCTACCCTGTGACTCCAGGCACGTC
231552_ENSRNOG00000007152_RAT_9344_9411/1-133       GGGAGCGAGTGGGTGGGTAGAAGCTACGTGT----TCTACCCTGTGACTCCAAGCACGTC
231552_ENSDARG00000004060_ZEBRAFISH_9664_9767/1-133 GAGTGTATGTGGGTGGATTTGAAGTACGTGTTAAATAAACCCTGTGACTGCAGACACGTC
231552_ENSMUSG00000030103_MOUSE_519_586/1-133       GGGAGCGAGTGGGTGGGTAGAAGCTACGTGT----TCTACCCTGTGACTCCAAGCACGTC
231552_SINFRUG00000133586_FUGU_2671_2800/1-133      -GGAAAGTGGGGGAGAACA-GAGCGACGAGTTAAATAAACCCTCTGACTCCGCGCAGCGC
                                                     .*:. .:* ***:*.. : .*.  ***:**    *.:***** ***** *. .**   *


231552_ENSG00000134107_HUMAN_7051_7118/1-133        TGGCC-ACTGCCG-----------------------------------------------
231552_ENSRNOG00000007152_RAT_9344_9411/1-133       TGGCC-GCTGCCG-----------------------------------------------
231552_ENSDARG00000004060_ZEBRAFISH_9664_9767/1-133 TCG-A-GCTCCAGTCACATGAGCCTCACGTGTGTGACGGCGCTTT---------------
231552_ENSMUSG00000030103_MOUSE_519_586/1-133       TGTCC-GCTANNN-----------------------------------------------
231552_SINFRUG00000133586_FUGU_2671_2800/1-133      TGACCTACCTCTGTCACATGAGGCTCACGTGTTAAACGGCGCTGTATCCCCGGCGGAGGG
                                                    *   . .*    .                                               


231552_ENSG00000134107_HUMAN_7051_7118/1-133        -------------
231552_ENSRNOG00000007152_RAT_9344_9411/1-133       -------------
231552_ENSDARG00000004060_ZEBRAFISH_9664_9767/1-133 ------------C
231552_ENSMUSG00000030103_MOUSE_519_586/1-133       -------------
231552_SINFRUG00000133586_FUGU_2671_2800/1-133      ACCGCCTCCTCC-
                                                                 

231552-rev.aln

RNAz output


############################  RNAz 0.1  ##############################

 Sequences: 5
 Columns: 133
 Mean pairwise identity:  49.43
 Mean single sequence MFE: -32.74
 Consensus MFE:  -9.26
 Energy contribution: -10.78
 Covariance contribution:   1.52
 Mean z-score:  -1.15
 Structure conservation index:   0.28
 SVM decision value:   0.34
 SVM RNA-class probability: 0.696569
 Prediction: RNA

######################################################################

>231552_ENSG00000134107_HUMAN_7051_7118/1-133
GGGAGCGAGUGGGUGGGUUGGAGCUACGUGUUCUACCCUGUGACUCCAGGCACGUCUGGCCACUGCCG
..(.((.(((((..((((.(((((.....))))))))).......(((((....))))))))))))). ( -26.10)
>231552_ENSRNOG00000007152_RAT_9344_9411/1-133
GGGAGCGAGUGGGUGGGUAGAAGCUACGUGUUCUACCCUGUGACUCCAAGCACGUCUGGCCGCUGCCG
((.((((.((.((.((((((((.(.....))))))))).(((........)))..)).)))))).)). ( -27.70)
>231552_ENSDARG00000004060_ZEBRAFISH_9664_9767/1-133
GAGUGUAUGUGGGUGGAUUUGAAGUACGUGUUAAAUAAACCCUGUGACUGCAGACACGUCUCGAGCUCCAGUCACAUGAGCCUCACGUGUGUGACGGCGCUUUC
((((.(((((((.(((((((((.(.((((((..........((((....)))))))))))))))).)))).))))))).(((((((....)))).))))))).. ( -36.90)
>231552_ENSMUSG00000030103_MOUSE_519_586/1-133
GGGAGCGAGUGGGUGGGUAGAAGCUACGUGUUCUACCCUGUGACUCCAAGCACGUCUGUCCGCUANNN
...((((..((((.((((((((.(.....))))))))).(((........))).))))..)))).... ( -21.30)
>231552_SINFRUG00000133586_FUGU_2671_2800/1-133
GGAAAGUGGGGGAGAACAGAGCGACGAGUUAAAUAAACCCUCUGACUCCGCGCAGCGCUGACCUACCUCUGUCACAUGAGGCUCACGUGUUAAACGGCGCUGUAUCCCCGGCGGAGGGACCGCCUCCUCC
(((..((.(((((.......(((..((((((...........)))))))))(((((((.......((((........))))....(((.....)))))))))).))))).))(((((.....)))))))) ( -51.70)
>consensus
GGGAGCGAGUGGGUGGGUAGGAGCUACGUGU____UCUACCCUGUGACUCCAAGCACGUCUGGCC_GCUGCCG____________________________________________________________
.((((((...(((((((.....((.....))....)))))))..)).)))).................................................................................. ( -9.26 = -10.78 +   1.52) 

231552-rev.rnaz

RNAalifold consensus structure

Secondary Structure Dot Plot
PS | PDF PS | PDF
Stefan Washietl
Last modified: Thu Aug 12 14:35:44 CEST 2004