CLUSTAL W(1.81) multiple sequence alignment 134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 AGCACCCCACTGCATTTCAATGTTTAA-ATTTTA-ATGAACCCTTTAAAAATGTCTCAAG 134270_ENSG00000164853_HUMAN_5374_7839/1-2687 --C--CGCGCCGC-GGCGGCTCCGTGC-GTCCCA-ATG--C---T--CGCATTTC-AATC 134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 AGCAGCCCTCTGTATTTCAGCGCGTGCAGCCCCACATT--C---TAACGAACTCCTCACA 134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 --CCGTGCGCCGCAGCCGGCTCCACGC-ATCTCA-ATG--C---G--ACCATTTC-AATC 134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 --CCGCGCGCTGCAGCCGGCTCCACGC-ATCTCA-ATG--C---G--ACCATTTC-AATC * * * * * ** * * * * 134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 ATGTAGCCTTTTAT--CCCTCTATCTTACAAAAAGTGCTTTTAGAAGTTTG--GGAATCT 134270_ENSG00000164853_HUMAN_5374_7839/1-2687 GCCCTGTGTTTT-TCATGCTGTCGGGCCGGCT-CGCGCTCCGA-AAACCTCCTCGAGTGT 134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 AATCTGCCTTAT-TCATCCTTCCTCCTCTTTTAAGCGCTT--C-TTTCTTC--GGATTT- 134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 GCCCAGTGGCNN-N--NNNNNNNNNNNNNNNN-NNNNCTC--A--------------TG- 134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 GCCCAGTGGCTT-T--TTNNNNNNNNNNNNNN-NNNNNNNNNN-NNNNNNNNNNNNNNNN * 134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 TTGAATGTTTTTTTCTTTGTGTCTTAAACTTAT--TTTAT-----CATACTGTACACAAG 134270_ENSG00000164853_HUMAN_5374_7839/1-2687 TTGAGAGGGGCCTTTAATGACGGTGGGGGCGGCCGGGACGACCCCCGC--CCGGCTCGAG 134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 -TGAGGGCGATCTG--ATGAATAATTGCCCCTC--TCTCG-----GTC--TCTTTTTGAA 134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 -TGCTCGGGACCCT--NNNNNNNNNNNNNNNTC--TCAAG-----CGT--TGGAGTGGGG 134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 GTGCCCGGGACCCTNNNNNNNNNNNNNNNNNNC--TCAAG-----CGT--TGGAGTGGGG ** * 134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 TATGAATAATTCGGTTTATCCAGATCCCCCTTTTGAACATCAGAATTCTATAATTGCAGC 134270_ENSG00000164853_HUMAN_5374_7839/1-2687 AGCTCTTCAGAGGCCCTCGGC-TGCGGCGGCGCCGCGGGGAGTCGCTCGGCTTCTGCTCT 134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 CATCTAAAATTCAGTGATTGCTTTTGGTCATTTTCAGGCACGACTCCAACCNNNNNNNNN 134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 GACTTTAATGACGGTGGGGGT-GGCCGAGTCGACCCCGCCCGGCTCGAGAGCTCTTCAGA 134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 GACTTTAATGACAGTGGGGGT-GGCCGAGACGACCCCGCCCGTCTCGAGAGCTCTTCAGA 134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 TTTGACAGTTCAGGGCGCGGTCTCGTCCTGGCGCGATCTTAGCTCAATGAAAAGC-AGCC 134270_ENSG00000164853_HUMAN_5374_7839/1-2687 CGGNNNNNNNN-NNNNNNNNNNNNNNNNNATTTTCAACATCAAAATTTCATAATCGCCTC 134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 NNNNNNNNNNN-NNNNNNNNNNNNNNNNNNNNNNN-NNNNNNNNNNNNNNNNNNNNNGTG 134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 GGCCCTCGGGC-TGGCGGTGGCGCGGCGGGGAGTC-ACTTGGCTCCAACTGGAGCGCGCA 134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 GGCCCTCGGGC-TGGCAGTGGCGCGGCGGGGAGTC-ACTTGGCTCCAACTGGAGCGCGCA
############################ RNAz 0.1 ############################## Sequences: 5 Columns: 300 Mean pairwise identity: 35.02 Mean single sequence MFE: -80.91 Consensus MFE: -8.17 Energy contribution: -9.70 Covariance contribution: 1.53 Mean z-score: -0.66 Structure conservation index: 0.10 SVM decision value: 0.06 SVM RNA-class probability: 0.561898 Prediction: RNA ###################################################################### >134270_ENSDARG00000020961_ZEBRAFISH_5965_8474/1-2687 AGCACCCCACUGCAUUUCAAUGUUUAAAUUUUAAUGAACCCUUUAAAAAUGUCUCAAGAUGUAGCCUUUUAUCCCUCUAUCUUACAAAAAGUGCUUUUAGAAGUUUGGGAAUCUUUGAAUGUUUUUUUCUUUGUGUCUUAAACUUAUUUUAUCAUACUGUACACAAGUAUGAAUAAUUCGGUUUAUCCAGAUCCCCCUUUUGAACAUCAGAAUUCUAUAAUUGCAGCUUUGACAGUUCAGGGCGCGGUCUCGUCCUGGCGCGAUCUUAGCUCAAUGAAAAGCAGCC .........((((.((((.((((((((((((..(((((((..(((((((((((....))))).(((((((................))))).))))))))((((((((((......(((.......)))......))))))))))......(((((((.......))))))).......)))))))..)))).......))))))))............((((.((((..((..((((((((((......)))))))).))..))..)))))))))))).)))).. ( -63.70) >134270_ENSG00000164853_HUMAN_5374_7839/1-2687 CCGCGCCGCGGCGGCUCCGUGCGUCCCAAUGCUCGCAUUUCAAUCGCCCUGUGUUUUUCAUGCUGUCGGGCCGGCUCGCGCUCCGAAAACCUCCUCGAGUGUUUGAGAGGGGCCUUUAAUGACGGUGGGGGCGGCCGGGACGACCCCCGCCCGGCUCGAGAGCUCUUCAGAGGCCCUCGGCUGCGGCGGCGCCGCGGGGAGUCGCUCGGCUUCUGCUCUCGGNNNNNNNNNNNNNNNNNNNNNNNNNAUUUUCAACAUCAAAAUUUCAUAAUCGCCUC ..((((((.(((((((((.((((((((((((....))))....((((((((((.....)))(((((((((((..(((((((((.((........))))))))..)))...))))).....)))))).)))))))..))))).....(((((((((.((((.((((....)).)).)))))))).)))))....))).)))))))))))))..(((....)))...................................................))... ( -106.30) >134270_SINFRUG00000149320_FUGU_3315_5852/1-2687 AGCAGCCCUCUGUAUUUCAGCGCGUGCAGCCCCACAUUCUAACGAACUCCUCACAAAUCUGCCUUAUUCAUCCUUCCUCCUCUUUUAAGCGCUUCUUUCUUCGGAUUUUGAGGGCGAUCUGAUGAAUAAUUGCCCCUCUCUCGGUCUCUUUUUGAACAUCUAAAAUUCAGUGAUUGCUUUUGGUCAUUUUCAGGCACGACUCCAACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGUG .((.((((((........((((((((......))).(((....)))..........................................)))))....((....))....))))))...(((((((((....(((........))).((.....)).........)))))((((((......))))))..))))))............................................................................... ( -38.60) >134270_ENSRNOG00000001284_RAT_5040_7483/1-2687 CCGUGCGCCGCAGCCGGCUCCACGCAUCUCAAUGCGACCAUUUCAAUCGCCCAGUGGCNNNNNNNNNNNNNNNNNNNNNNNCUCAUGUGCUCGGGACCCUNNNNNNNNNNNNNNNUCUCAAGCGUUGGAGUGGGGGACUUUAAUGACGGUGGGGGUGGCCGAGUCGACCCCGCCCGGCUCGAGAGCUCUUCAGAGGCCCUCGGGCUGGCGGUGGCGCGGCGGGGAGUCACUUGGCUCCAACUGGAGCGCGCA ...(((((....((((.((((((((((....))))..((((((((((.(((....)))..............................(((.((((...................)))).)))))))))))))...............)))))).))))(((((.((((((((((((((((((.((((....)).)).)))))))))(((....))))))))))..)))))))(((((....)))))))))) ( -100.31) >134270_ENSMUSG00000029546_MOUSE_5013_7498/1-2687 CCGCGCGCUGCAGCCGGCUCCACGCAUCUCAAUGCGACCAUUUCAAUCGCCCAGUGGCUUUUUNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGUGCCCGGGACCCUNNNNNNNNNNNNNNNNNNCUCAAGCGUUGGAGUGGGGGACUUUAAUGACAGUGGGGGUGGCCGAGACGACCCCGCCCGUCUCGAGAGCUCUUCAGAGGCCCUCGGGCUGGCAGUGGCGCGGCGGGGAGUCACUUGGCUCCAACUGGAGCGCGCA ..(((((((.((((((.(.((((((((....))))(.(((........(((....)))...............................................((((((((((((....................((..(((((((((.....)))))))))....)))))))((((((((((........))))))(((.....)))...))))))))))))))).)))).)))))..((((((....))))))...)).))))))). ( -95.65) >consensus __CAGCGCGCUGCAGCCGGCUCCAUGC_AUCUCA_AUG__C___U__ACCAUUUC_AAUCGCCCAGUGUUUU_U__UCCU______________G_GCUC__A______U____GA_UG__UGAGCGGGACCUU___UG____________UC__UCAAG_____CGU__UGGACUCGAGGACUUUAAAGACGGUGGGGGC_GGCCGAGACGACCCCGCCCGGCUCGAGACCUCUGCAGAGGC____G__C__GGC___G_C_CG_C__GGAGUC_ACUUGGCUCCAACAGAAGCGCGCA ....((((((...................................................................................................................((((((.............................................((....)).........((((((..............))))))..))))))..........................................(((((.......))))).......)))))). ( -8.17 = -9.70 + 1.53)
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