Documentation
Manual Pages
- ncDNAlign.1.cutSequences.pl
- ncDNAlign.2.getGwAln.pl
- ncDNAlign.3.mergeGwAln.pl
- ncDNAlign.4.realign.pl
- ncDNAlign.5.trimAln.pl
getGenomewideAln.pl
NAME
getGenomewideAln.pl
- part of the NcDNAlign alignment pipeline, step (2)
Wraps a pairwise BLAST search of the target genome against all other organisms listed in the configurations file.
SYNOPSIS
getGenomewideAln.pl [options]
OPTIONS
- -c, --conf FILE
-
Path to central NcDNAlign configuration file
- -s, --silent [0|1]
-
Silent mode, avoid printing to STDOUT 0=OFF (Default), 1=ON
- -v, --version
-
Prints version information and exits.
- -h, --help
-
Prints a short help message and exits.
- --man
-
Prints a detailed manual page and exits.
DESCRIPTION
The script performs a pairwise nucleotide BLAST search using BLASTN to align the reference sequence heuristically against all other species. The best hit for each query subsequence is retained if the EVALUE of the BLAST search does not exceed the EVALUE-threshold and the query length is larger than a certain minimal length. Both thresholds are given in the config file. Results of the BLAST search are stored at the directory 'blast'.
EXAMPLES
$ getGenomewideAln.pl -c config-file.cfg
AUTHORS
Dominic Rose (dominic@bioinf.uni-leipzig.de)