species group genome size (Gb)* % mC/C % mC/CG*** references for mC/C content**** Odontophrynus americanus Amphibia 3.25 7.78 n.a. Jabbari et al. 1997 Rana pipiens Amphibia 6.48 9.73 n.a. Jabbari et al. 1997 Rana temporaia Amphibia 4.49 10.9 n.a. Vanyushin et al. 1973 Xenopus laevis Amphibia 3.15 6.64 95.68 Jabbari et al. 1997 Lampetra fluviatilis Cyclostomata 1.37 8.21 n.a. Vanyushin et al. 1973 Acheta domestica Invertebrata 2.03 0.28 n.a. this study Acyrthosiphon pisum Invertebrata 0.31 0.69 3.67 Walsh et al. 2010 Aedes albopictus Invertebrata 1.12 0.17 0.9 Regev et al. 1998 Apis mellifera Invertebrata 0.19 0.28 1.03 Zemach et al. 2010 Bombyx mori Invertebrata 0.53 0.24 1.12 Zemach et al. 2010 Caenorhabditis elegans Invertebrata 0.1 0.01 0.06 Simpson et al. 1986 Chorthippus parallelus Invertebrata 13.26 4.06 n.a. this study Ciona intestinalis Invertebrata 0.2 4.7 30.09 Zemach et al. 2010 Cucumaria frondosa Invertebrata 3.1** 5.75 n.a. Vanyushin et al. 1973 Daphnia magna Invertebrata 0.47 0.47 2.21 Vandegehuchte et al. 2009 Drosophila melanogaster Invertebrata 0.18 0.11 0.56 Zemach et al. 2010 Dysidea avara Invertebrata 0.06** 7.9 n.a. Regev et al. 1998 Gryllotalpa fossor Invertebrata 0.85 3 n.a. Sarkar et al. 1992 Helix pomatia Invertebrata 4** 2.9 n.a. Regev et al. 1998 Hydra magnipapillata Invertebrata 1.29** 2.85 25.61 Hassel et al. 2010 Locusta migratoria Invertebrata 6.09 1.25 n.a. this study Marmorkrebs (Procambarus) Invertebrata 5.33** 1.64 n.a. Schiewek et al. 2007 Nematostella vectensis Invertebrata 0.23 1.8 11.31 Zemach et al. 2010 Nephtys ciliata Invertebrata 4.7** 8.4 n.a. Regev et al. 1998 Paracentrotus lividus Invertebrata 0.72 6.6 n.a. Regev et al. 1998 Pecten islandicus Invertebrata 1.76** 2.5 n.a. Regev et al. 1998 Schistosoma mansoni Invertebrata 0.26 0 0 Fantappie et al. 2001 Stichopus japonicus Invertebrata 0.89** 3.28 n.a. Vanyushin et al. 1973 Suberites domuncula Invertebrata 1.6 6.7 n.a. Regev et al. 1998 Tribolium castaneum Invertebrata 0.21 0.12 0.61 Zemach et al. 2010 Bos taurus Mammalia 3.65 5.93 121.41 Romanov and Vanyushin 1981 Canis familiaris Mammalia 3.14 3.26 60.82 Jabbari et al. 1997 Cavia porcellus Mammalia 3.78 3.73 87.35 Jabbari et al. 1997 Equus caballus Mammalia 3.18 4.86 81.96 Jabbari et al. 1997 Erinaceus europaeus Mammalia 3.66 2.33 54.31 Jabbari et al. 1997 Hapalemur griseus Mammalia 3.22 4.49 n.a. Jabbari et al. 1997 Homo sapiens Mammalia 3.5 4.52 93.67 Rossella et al. 2009 Macaca fascicularis Mammalia 3.43 4.65 n.a. Gama-Sosa et al. 1983 Macaca mulatta Mammalia 3.37 4.75 92.59 Grunau et al. 2001 Mesocricetus auratus Mammalia 3.49 2.25 n.a. Wilson and Jones 1983 Mus musculus Mammalia 3.3 4.37 110.08 Grunau et al. 2001 Ornithorhynchus anatinus Mammalia 3.06 5.79 67.48 Jabbari et al. 1997 Oryctolagus cuniculus Mammalia 3.26 3.01 47.78 Grunau et al. 2001 Ovis aries Mammalia 3.3 4.47 n.a. Jabbari et al. 1997 Procavia capensis Mammalia 4.06 3.41 n.a. Jabbari et al. 1997 Pteropus poliocephalus Mammalia 2.32 6.7 n.a. Jabbari et al. 1997 Rattus norvegicus Mammalia 3.27 4.39 95.23 Grunau et al. 2001 Saimiri sciureus Mammalia 3.3 4.55 n.a. Gama-Sosa et al. 1983 Sus scrofa Mammalia 3.09 4.58 81.64 Jabbari et al. 1997 Tachyglossus aculeatus Mammalia 2.89 4.31 n.a. Jabbari et al. 1997 Acipenser gueldenstaedtii Osteichthyes 3.94 8.17 n.a. Vanyushin et al. 1973 Acipenser ruthenus Osteichthyes 1.87 8.7 n.a. Vanyushin et al. 1973 Acipenser stellatus Osteichthyes 2.35 8.72 n.a. Vanyushin et al. 1973 Boops boops Osteichthyes 0.74 8.2 n.a. Varriale & Bernardi 2006 Boreogadus saida Osteichthyes 0.88 9.16 n.a. Varriale & Bernardi 2006 Carrassius auratus Osteichthyes 1.78 7.28 n.a. Varriale & Bernardi 2006 Chionodraco hamatus Osteichthyes 1.83 10.94 n.a. Varriale & Bernardi 2006 Clupea harengus Osteichthyes 0.94** 8.19 n.a. Vanyushin et al. 1973 Coris julis Osteichthyes 1.21 5.7 n.a. Varriale & Bernardi 2006 Cyprinus carpio Osteichthyes 1.79 6.43 n.a. Vanyushin et al. 1973 Danio rerio Osteichthyes 1.78 6.9 70.55 Varriale & Bernardi 2006 Dissostichus mawsoni Osteichthyes 1.02 8.4 n.a. Varriale & Bernardi 2006 Esox lucius Osteichthyes 1.13 6.76 n.a. Vanyushin et al. 1973 Fundulus heteroclitus Osteichthyes 1.36 8.22 n.a. Varriale & Bernardi 2006 Gadus morhua Osteichthyes 0.67 8.89 n.a. Vanyushin et al. 1973 Huso huso Osteichthyes 1.51 8.34 n.a. Vanyushin et al. 1973 Ictalurus nebulosus Osteichthyes 1.2 9.3 n.a. Jabbari et al. 1997 Liparis tunicatus Osteichthyes 0.88 12.04 n.a. Varriale & Bernardi 2006 Mugil cephalus Osteichthyes 0.81 9.29 n.a. Jabbari et al. 1997 Onchorhynchus keta Osteichthyes 2.64 6.5 n.a. Varriale & Bernardi 2006 Onchorhynchus mykiss Osteichthyes 2.75 6.76 n.a. Jabbari et al. 1997 Onchorhynchus tshawytscha Osteichthyes 2.58 8.26 n.a. Jabbari et al. 1997 Oreochromis niloticus Osteichthyes 1.12 6.15 n.a. Jabbari et al. 1997 Oryzias latipes Osteichthyes 1.08 9.07 92.74 Jabbari et al. 1997 Osmerus eperlanus Osteichthyes 0.62 8.85 n.a. Vanyushin et al. 1973 Pantodon buchholzi Osteichthyes 0.77 7.76 n.a. Varriale & Bernardi 2006. MethDB Paralichthys californicus Osteichthyes 0.8 6.8 n.a. Varriale & Bernardi 2006 Pleuronectes flesus Osteichthyes 0.55 7.57 n.a. Vanyushin et al. 1973 Silurus glanis Osteichthyes 1.23 7.92 n.a. Vanyushin et al. 1973 Symphodus tinca Osteichthyes 0.73 8.02 n.a. Varriale & Bernardi 2006 Tetraodon nigroviridis Osteichthyes 0.43 10.7 76.37 Zemach et al. 2010 Thunnus thynnus Osteichthyes 0.8 8.32 n.a. Vanyushin et al. 1973 Trematomus bernacchii Osteichthyes 1.51 8.34 n.a. Varriale & Bernardi 2006 Xiphophorus maculatus Osteichthyes 0.94 6.74 n.a. Varriale & Bernardi 2006 Alligator mississippiensis Sauropsida 2.58 3.94 n.a. Varriale & Bernardi 2006 Angius fragilis Sauropsida 2.35 4.22 n.a. Varriale & Bernardi 2006 Boa constrictor Sauropsida 2.04 3.24 n.a. Varriale & Bernardi 2006 Bothrops jacaraca Sauropsida 2.25 5.4 n.a. Varriale & Bernardi 2006 Cairina moschata Sauropsida 1.28 3.08 n.a. Jabbari et al. 1997 Chelonia mydas Sauropsida 2.64 4.2 n.a. Varriale & Bernardi 2006 Chelydra serpentina Sauropsida 2.61 5.58 n.a. Varriale & Bernardi 2006 Chrysemis picta Sauropsida 2.61 4.04 n.a. Varriale & Bernardi 2006 Columba livia Sauropsida 1.48 4.64 n.a. Vanyushin et al. 1973 Corvus corone Sauropsida 1.35 5.07 n.a. Vanyushin et al. 1973 Crocodylus niloticus Sauropsida 3.39 3.5 n.a. Varriale & Bernardi 2006 Elaphe obsoleta Sauropsida 2.18 5.5 n.a. Varriale & Bernardi 2006 Gallus gallus Sauropsida 1.25 3.78 72.69 Grunau et al. 2001 Gekko gekko Sauropsida 2.84 4.74 n.a. Varriale & Bernardi 2006 Iguana iguana Sauropsida 2.89 6.16 n.a. Varriale & Bernardi 2006 Natrix tesselata Sauropsida 1.91 4.64 n.a. Varriale & Bernardi 2006 Podarcis muralis Sauropsida 1.7 5.58 n.a. Varriale & Bernardi 2006 Podarcis sicula Sauropsida 2.2 6.26 n.a. Varriale & Bernardi 2006 Python reticulatis Sauropsida 1.6 3.08 n.a. Varriale & Bernardi 2006 Sceloporus magister Sauropsida 2.73 3.7 n.a. Varriale & Bernardi 2006 Spheniscus demersus Sauropsida 1.63 4.67 n.a. Jabbari et al. 1997 Tarentola mauritanica Sauropsida 2.65 4.02 n.a. Varriale & Bernardi 2006 Testudo graeca Sauropsida 5.44 3.37 n.a. Jabbari et al. 1997 Testudo horsfeldi Sauropsida 3.52** 5.66 n.a. Vanyushin et al. 1973 Trachemys scripta elegans Sauropsida 2.59 4.86 n.a. Varriale & Bernardi 2006 Tupinambis teguixin Sauropsida 2.53 3.76 n.a. Varriale & Bernardi 2006 Varanus griseus Sauropsida 1.19 5.26 n.a. Vanyushin et al. 1973 Viper aspis francisredi Sauropsida 2.91 5.52 n.a. Varriale & Bernardi 2006 * genome sizes are from Gregory 2010 ** genome size medians from other species of the genus *** mC/CG data were determined using the CG dinucleotide content of genome sequences **** Sources of mC/C: "Fantappi MR, Gimba ER, Rumjanek FD. Lack of DNA methylation in Schistosoma mansoni. Exp Parasitol. 2001 Jul;98(3):162-6." "Gama-Sosa MA, MIdgett RM, Slagel VA, Githens S, Kuo KC, Gehrke CW, Ehrlich M Tissue-specific differences in DNA methylation in various mammals. Biochim Biophys Acta. 1983 Jun 24;740(2):212-9." Gregory, T.R. (2011). Animal Genome Size Database. http://www.genomesize.com. "Grunau C, Renault E, Rosenthal A, Roizes G. MethDB--a public database for DNA methylation data. Nucleic Acids Res. 2001 Jan 1;29(1):270-4." "Hassel M, Cornelius MG, Vom Brocke J, Schmeiser HH. Total nucleotide analysis of Hydra DNA and RNA by MEKC with LIF detection and 32P-postlabeling. Electrophoresis. 2010 Jan;31(2):299-302." "Jabbari K, Cacci S, Pas de Barros JP, Desgrs J, Bernardi G. Evolutionary changes in CpG and methylation levels in the genome of vertebrates. Gene. 1997 Dec 31;205(1-2):109-18." Regev A, Lamb M, Jablonka E, 1998. The role of DNA methylation in invertebrates: Developmental regulation or genome defense? Mol Biol Evol 15:880891. "Romanov GA, Vanyushin BF Methylation of reiterated sequences in mammalian DNAs. Effects of the tissue type, age, malignancy and hormonal induction. Biochim Biophys Acta. 1981 Apr 27;653(2):204-18." Rossella F, Polledri E, Bollati V, Baccarelli A, Silvia Fustinoni S. Development and validation of a gas chromatography/ mass spectrometry method for the assessment of genomic DNA methylation. Rapid Commun Mass Spectrom, 2009, 23 (17) pp. 2637-46. "Sarkar S, Rao SR. Insect sex chromosomes, XI. 3H-TdR induces random aberrations in the X chromosome(s) of Gryllotalpa fossor (Orthoptera). Mutat Res. 1992 Jun;282(2):113-7." "Schiewek R, Wirtz M, Thiemann M, Plitt K, Vogt G, Schmitz OJ. Determination of the DNA methylation level of the marbled crayfish: an increase in sample throughput by an optimised sample preparation. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 1;850(1-2):548-52." "Simpson VJ, Johnson TE, Hammen RF. Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging. Nucleic Acids Res. 1986 Aug 26;14(16):6711-9." "Vandegehuchte MB, Kyndt T, Vanholme B, Haegeman A, Gheysen G, Janssen CR. Occurrence of DNA methylation in Daphnia magna and influence of multigeneration Cd exposure. Environ Int. 2009 May;35(4):700-6." "Vanyushin BF, Mazin AL, Vasilyev VK, Belozersky AN The content of 5-methylcytosine in animal DNA: the species and tissue specificity. Biochim Biophys Acta. 1973 Mar 28;299(3):397-403." Varriale A, Bernardi G, 2006a. DNA methylation and body temperature in fishes. Gene 385:111121. Varriale A, Bernardi G, 2006b. DNA methylation in reptiles. Gene 385:122127. "Walsh TK, Brisson JA, Robertson HM, Gordon K, Jaubert-Possamai S, Tagu D, Edwards OR. A functional DNA methylation system in the pea aphid, Acyrthosiphon pisum. Insect Mol Biol. 2010 Mar;19 Suppl 2:215-28." "Wilson VL, Jones PA DNA methylation decreases in aging but not in immortal cells. Science. 1983 Jun 3;220(4601):1055-7." "Wilson VL, Smith RA, Autrup H, Krokan H, Musci DE, Le NN, Longoria J, Ziska D, Harris CC. Genomic 5-methylcytosine determination by 32P-postlabeling analysis. Anal Biochem. 1986 Feb 1;152(2):275-84." Zemach A, McDaniel IE, Silva P, Zilberman D, 2010. Genome-wide evolutionary analysis of eukaryotic DNA methylation. Science 328:916919.