5 81 1234567890 Dr_C12b ATCCTCAACGAGTTCATCACCAGACAGCGGAGGAGGGAACTGTCTGACCGGCTAAATCTCACGGACCAACAAGTGAAAATC Tn_C12a ATCATGAACGAGTTCATCACCAGGCAGCGGCGGAGGGAGCTGTCCGATCGCCTGAACCTCAGCGACCAACAGGTCAAGATC Dr_D12a ------AACGAATTCATGACGAGGCAGAAACGGAAGGAGCTTTCGGACAGATTGGAACTGAGTGATCAGCAAGTCAAAATC Tn_D12a CTGATGAATGAGTTCATCAACAGGCAGAAACGGTAGGAACTCTCCGACAGGCTGGACCTGAGCGACCAGCAGGTCAAAATC Tr_D12a CTGATGAATGAGTTCATCAACAGACAGAAACGGAAGGAGCTCTCCGACAGGCTGGACTTGAGCGACCAGCAAGTAAAAATC const .*..*.**.**.*******..**.***....**..***.**.**.**..*..*..*..*.*..**.**.**.**.**.*** SplitCD #.#........................###....#.............#.....#....#........#............ MF-12-1 --------------------------GCGGCGGAGGGAGCTCTCCGATCGGCTGAACCTCAGCGACCAACAAGTCAAGATC ...........................CCC....C.............C.....C....C........C............ ..............................t..*....t..x..t..t..d..t..t*...tt..*.....d..t..t... No positions that is constant in the Fugu/Danio/Tetraodon comparison is different in MF-12-1. In all nucleotides that distinguish C from D cluster sequences MF-12-1 follows the C-cluster. It is most closely related with the tetraodon sequence Conclusion: MF-12-1 is a HoxC12a gene.