AAAUGUUAACAAAUUUGGCAGU_UA_CUUUGGAUCUAUCACCUGUCAUCAUAACUGGCUGCUGCUU____GUCAUCCA_CAC_AACACCAGGACUUAGGACAAAUGG______GACUGAUGUCAUCUUGAGCUCU__UCA_UUUAUUUUGACCGU_GAUUUAUUUGGAGUGGAGGCAUUGUUUUU_______AAAAAAACA_________UGUCAUGUAGGUUGUCUAAAAAUAAAAUGCAUUUAAACUCAUUUGAGAG
...........................(((((((.....(((((.(((.(((....(((.((((((......................(((..((..........))........)))...(((...((((..........))))...)))...............)))))).)))..(((((((.........))))))).........))).)))))))).))))))).............................
 minimum free energy = -19.55 kcal/mol (-19.90 +   0.35)