FUGUE v2.s.07 (01 Apr 2003)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 5245
Probe sequence ID : t0253
Probe sequence len : 194
Probe divergence : 0.548
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
hsd1qb7a 236 202 416 27.64 29.62 00 CERTAIN Alignment hs1g2qa 178 240 364 26.60 28.58 00 CERTAIN Alignment hs1o57a 270 64 388 24.32 26.41 00 CERTAIN Alignment hs1o57a 270 64 388 24.32 26.41 00 CERTAIN Alignment hsd1oroa 213 -57 168 8.80 11.02 00 CERTAIN Alignment hs1ufra 164 -65 124 8.09 10.31 00 CERTAIN Alignment GATase_2 508 -654 111 8.06 10.27 03 CERTAIN Alignment prt 226 -128 112 6.65 9.03 00 CERTAIN Alignment hsd1bd3a 224 -138 77 4.69 6.82 00 LIKELY Alignment hs1dkua 295 -264 68 3.56 5.46 03 MARGINAL Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score hsd1qb7a aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 27.64 hs1g2qa aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 26.60 hs1o57a aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 24.32 hs1o57a aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 24.32 hsd1oroa aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 8.80 hs1ufra aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 8.09 GATase_2 aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 8.06 prt aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 6.65 hsd1bd3a aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 4.69 hs1dkua aa ma mh hh aa ma mh hh aa ma mh hh MARGINAL PDB Chime 3.56Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk