FUGUE v2.s.07 (01 Apr 2003)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 5245
Probe sequence ID : t0268
Probe sequence len : 285
Probe divergence : 0.583
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
hs1m6ya 289 725 1011 50.26 52.46 00 CERTAIN Alignment hs1l3ia 178 -147 123 8.19 10.68 02 CERTAIN Alignment hs1ej0a 180 -79 67 5.46 7.69 22 LIKELY Alignment hs1pjza 201 -167 101 4.79 7.25 00 LIKELY Alignment hs1ri5a 252 -196 77 4.54 7.05 00 LIKELY Alignment hs1lsua 134 -178 51 3.69 6.17 02 MARGINAL Alignment hs1vl5a 225 -157 89 3.54 5.71 00 MARGINAL Alignment hsd1xvaa 292 -250 74 3.18 5.65 00 GUESS Alignment hs3thia 362 -255 64 2.98 4.00 00 GUESS Alignment hsd1thta 294 -171 53 2.95 4.75 00 GUESS Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score hs1m6ya aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 50.26 hs1l3ia aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 8.19 hs1ej0a aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 5.46 hs1pjza aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 4.79 hs1ri5a aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 4.54 hs1lsua aa ma mh hh aa ma mh hh aa ma mh hh MARGINAL PDB Chime 3.69 hs1vl5a aa ma mh hh aa ma mh hh aa ma mh hh MARGINAL PDB Chime 3.54 hsd1xvaa aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 3.18 hs3thia aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.98 hsd1thta aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.95Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk