FUGUE v2.s.07 (01 Apr 2003)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 4555
Probe sequence ID : T0157
Probe sequence len : 138
Probe divergence : 0.722
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
hsd1hjra 158 -60 111 5.06 7.57 00 LIKELY Alignment hs1gsoa 419 -627 58 4.06 6.75 03 LIKELY Alignment hs1o9ga 247 -252 39 3.70 4.99 03 MARGINAL Alignment MutS_D2 170 -151 70 2.99 5.76 00 GUESS Alignment hsd4crxa1 110 -61 53 2.48 3.77 00 GUESS Alignment hs1l7ya 94 -65 36 2.19 3.80 00 GUESS Alignment hs1im4a 205 -200 44 1.99 4.71 00 UNCERTAIN Alignment hs1m4ua 199 -177 46 1.98 4.15 00 UNCERTAIN Alignment hs1cfza 162 -152 49 1.95 4.66 00 UNCERTAIN Alignment hs1el6a 208 -147 36 1.93 3.22 03 UNCERTAIN Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score hsd1hjra aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 5.06 hs1gsoa aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 4.06 hs1o9ga aa ma mh hh aa ma mh hh aa ma mh hh MARGINAL PDB Chime 3.70 MutS_D2 aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.99 hsd4crxa1 aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.48 hs1l7ya aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.19 hs1im4a aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.99 hs1m4ua aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.98 hs1cfza aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.95 hs1el6a aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.93Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk