FUGUE v2.s.07 (01 Apr 2003)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 4555
Probe sequence ID : t0146
Probe sequence len : 325
Probe divergence : 0.730
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
hs1kafa 108 -250 28 1.96 3.27 02 UNCERTAIN Alignment hsd1prea1 83 -274 25 1.93 3.94 02 UNCERTAIN Alignment hs1kj6a 45 -305 19 1.82 3.18 02 UNCERTAIN Alignment hsd1regx 122 -239 47 1.82 3.55 02 UNCERTAIN Alignment hs1crza 397 -510 44 1.81 4.65 00 UNCERTAIN Alignment hs1cfnc 9 -305 23 1.81 3.12 02 UNCERTAIN Alignment hs1kcna 21 -313 10 1.80 3.11 02 UNCERTAIN Alignment hsd1kp6a 79 -314 27 1.80 3.11 02 UNCERTAIN Alignment hs1jlpa 20 -321 21 1.76 3.07 02 UNCERTAIN Alignment hs1izlk 27 -286 17 1.71 3.02 02 UNCERTAIN Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score hs1kafa aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.96 hsd1prea1 aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.93 hs1kj6a aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.82 hsd1regx aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.82 hs1crza aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.81 hs1cfnc aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.81 hs1kcna aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.80 hsd1kp6a aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.80 hs1jlpa aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.76 hs1izlk aa ma mh hh aa ma mh hh aa ma mh hh UNCERTAIN PDB Chime 1.71Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk