FUGUE v2.s.07 (01 Apr 2003)
Search sequence(s) against fold library using environment-specific
substitution tables and structure-dependent gap penalties.
Fold library and substitution tables are based on the HOMSTRAD database.
http://www-cryst.bioc.cam.ac.uk/~homstrad/
FUGUE server is available at:
http://www-cryst.bioc.cam.ac.uk/~fugue/
http://www-cryst.bioc.cam.ac.uk/~fugue/prfsearch.html
Citation:
J. Shi, T. L. Blundell and K. Mizuguchi (2001), J Mol Biol, 310(1), 243-57
Size of fold library: 4555
Probe sequence ID : TARGET
Probe sequence len : 251
Probe divergence : 0.320
Recommended cutoff : ZSCORE >= 6.0 (CERTAIN 99% confidence)
Other cutoff : ZSCORE >= 4.0 (LIKELY 95% confidence)
Other cutoff : ZSCORE >= 3.5 (MARGINAL 90% confidence)
Other cutoff : ZSCORE >= 2.0 (GUESS 50% confidence)
Other cutoff : ZSCORE < 2.0 (UNCERTAIN)
PLEN : Profile length
RAWS : Raw alignment score
RVN : (Raw score)-(Raw score for NULL model)
ZSCORE : Z-score normalized by sequence divergence
ZORI : Original Z-score (before normalization)
AL : Alignment algorithm used for Zscore/Alignment calculation
0 -- Global, 2 -- GloLocSeq (No sequence termini gap penalty)
3 -- GloLocPrf (No profile termini gap penalty)
The sequence(s) you submitted is HERE (in original format).
The sequence(s) actually used by FUGUE is HERE (in PIR format).
Download all the results in compressed format HERE. new!
View Ranking (Click on a profile hit will bring you to the corresponding HOMSTRAD family)
Profile Hit PLEN RAWS RVN ZSCORE ZORI AL
Alpha_adaptinC12 262 -98 124 7.93 9.86 00 CERTAIN Alignment hs1e42a 233 -41 145 4.99 6.49 00 LIKELY Alignment hs1nbwa 606 -717 58 3.80 4.96 03 MARGINAL Alignment hsd1knya 253 -104 63 2.74 3.23 00 GUESS Alignment hsd1b35c 282 -198 36 2.63 4.56 00 GUESS Alignment hs1iu1a 120 -129 34 2.49 4.42 02 GUESS Alignment hs1dkia 303 -207 79 2.44 3.85 00 GUESS Alignment hs1ikta 115 -40 17 2.43 4.33 22 GUESS Alignment hs1qu6a 179 -142 40 2.33 4.41 00 GUESS Alignment hsd1ema 221 -153 60 2.03 3.69 00 GUESS Alignment
View Alignments (What do they mean -- aa, ma, mh, hh? See note at the bottom of the page.)
Hint: check 'ma' first if your query is a single sequence, otherwise start with 'aa'. Profile Hit HTML POSTSCRIPT TEXT(PIR FORMAT) Confidence View Model Z-score Alpha_adaptinC12 aa ma mh hh aa ma mh hh aa ma mh hh CERTAIN PDB Chime 7.93 hs1e42a aa ma mh hh aa ma mh hh aa ma mh hh LIKELY PDB Chime 4.99 hs1nbwa aa ma mh hh aa ma mh hh aa ma mh hh MARGINAL PDB Chime 3.80 hsd1knya aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.74 hsd1b35c aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.63 hs1iu1a aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.49 hs1dkia aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.44 hs1ikta aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.43 hs1qu6a aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.33 hsd1ema aa ma mh hh aa ma mh hh aa ma mh hh GUESS PDB Chime 2.03Keys
aa: query sequences (including PSI-BLAST homologues) aligned against all the representative structures from a HOMSTRAD family ma: master sequence aligned against all the representative structures from a HOMSTRAD family mh: master sequence aligned against a single structure of highest sequence identity from a HOMSTRAD family hh: single sequence/structure pair with highest sequence identity in 'aa' Note: If your query is a single sequence, master sequence is equivelent to your query and all the other sequences (if any) are collected by PSI-BLAST. If your query is a sequence alignment, master sequence is set to the first sequence in the alignment.JOY Keys are described here
If you have any further questions, please write to Jiye Shi and Kenji Mizuguchi at fugue@cryst.bioc.cam.ac.uk